ArticleProceedings of the National Academy of Sciences of the United States of America2003
Purifying mRNAs with a high-affinity eIF4E mutant identifies the short 3' poly(A) end phenotype.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 68 citations in OpenAlex.
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- Immunoprecipitation Methods to Isolate Messenger Ribonucleoprotein Complexes (mRNP).Advances in experimental medicine and biology · 2024Article
- Plant terminators: the unsung heroes of gene expression.Journal of experimental botany · 2023Review
- mRNA-based modalities for infectious disease management.Nano research · 2023Review
- Measuring the tail: Methods for poly(A) tail profiling.Wiley interdisciplinary reviews. RNA · 2023Review
- A cap 0-dependent mRNA capture method to analyze the yeast transcriptome.Nucleic acids research · 2022Article
- mRNA-based therapeutics: powerful and versatile tools to combat diseases.Signal transduction and targeted therapy · 2022Review
- S-adenosylmethionine-dependent methyltransferase inhibitor DZNep blocks transcription and translation of SARS-CoV-2 genome with a low tendency to select for drug-resistant viral variants.Antiviral research · 2022Article
- Global approaches for profiling transcription initiation.Cell reports methods · 2021Review
- Enhanced Prodigiosin Production inApplied and environmental microbiology · 2021Article
- To cap it all off, again: dynamic capping and recapping of coding and non-coding RNAs to control transcript fate and biological activity.Cell cycle (Georgetown, Tex.) · 2021Review
- High resolution biosensor to test the capping level and integrity of mRNAs.Nucleic acids research · 2020Article
- Immunoglobulin expression and the humoral immune response is regulated by the non-canonical poly(A) polymerase TENT5C.Nature communications · 2020Article
- Functional characterization of the translation initiation factor eIF4E of Echinococcus granulosus.Parasitology research · 2019Article
- Tales of Detailed Poly(A) Tails.Trends in cell biology · 2019Review
- RNA-Seq-Based Analysis Reveals Heterogeneity in Mature 16S rRNA 3' Termini and Extended Anti-Shine-Dalgarno Motifs in Bacterial Species.G3 (Bethesda, Md.) · 2018Article
- Dicer cleaves 5'-extended microRNA precursors originating from RNA polymerase II transcription start sites.Nucleic acids research · 2018Article
- Rice Stripe Tenuivirus Has a Greater Tendency To Use the Prime-and-Realign Mechanism in Transcription of Genomic than in Transcription of Antigenomic Template RNAs.Journal of virology · 2018Article
- Global characterization of the Dicer-like protein DrnB roles in miRNA biogenesis in the social amoeba Dictyostelium discoideum.RNA biology · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The use of DNA microarrays has revolutionized the manner in which mRNA populations are analyzed. One limitation of the current technology is that mRNAs are often purified on the basis of their 3' poly(A) ends, which can be extremely short or absent in some mRNAs. To circumvent this limitation, we have developed a procedure for the purification of eukaryotic mRNAs using a mutant version of the mRNA 5' cap-binding protein (eIF4E) with increased affinity for the m7GTP moiety of the cap. By using this procedure, we have compared the populations of mammalian mRNAs purified by oligo(dT) and 5' cap selection with oligonucleotide microarrays. This analysis has identified a subpopulation of mRNAs that are present with short 3' poly(A) ends at steady state and are missed or underrepresented after purification by oligo(dT). These mRNAs may respond to specific posttranscriptional control mechanisms such as cytoplasmic polyadenylation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.