ArticleJournal of bacteriology2003
Molecular characterization of inulosucrase from Leuconostoc citreum: a fructosyltransferase within a glucosyltransferase.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 92 citations in OpenAlex.
- Cold induced expression of a novel levansucrase gene sacB1 enhances exopolysaccharide production and stress resilience in Leuconostoc mesenteroides.Scientific reports · 2025Article
- Multidomain chimeric enzymes as a promising alternative for biocatalysts improvement: a minireview.Molecular biology reports · 2024Review
- Analysing the dynamics of the bacterial community in pozol, a Mexican fermented corn dough.Microbiology (Reading, England) · 2023Article
- Structure-Function Relationship Studies of Multidomain Levansucrases from Leuconostocaceae Family.Microorganisms · 2022Article
- Characterization and Antioxidant Activity of Released Exopolysaccharide from Potential ProbioticJournal of microbiology and biotechnology · 2021Article
- Functional and Molecular Characterization of theMicroorganisms · 2021Article
- Extracellular polysaccharides produced by bacteria of the Leuconostoc genus.World journal of microbiology & biotechnology · 2020Review
- Evolution of Fructans inFrontiers in nutrition · 2020Article
- Draft Genome Sequence ofGenome announcements · 2017Article
- Characterization of the First α-(1→3) Branching Sucrases of the GH70 Family.The Journal of biological chemistry · 2016Article
- Synthesis of Fructooligosaccharides by IslA4, a truncated inulosucrase from Leuconostoc citreum.BMC biotechnology · 2015Article
- Biopolymers from lactic acid bacteria. Novel applications in foods and beverages.Frontiers in microbiology · 2015Review
- Design of chimeric levansucrases with improved transglycosylation activity.Applied and environmental microbiology · 2012Article
- A novel dextransucrase is produced by Leuconostoc citreum strain B/110-1-2: an isolate used for the industrial production of dextran and dextran derivatives.Journal of industrial microbiology & biotechnology · 2011Article
- Gene cloning and enzyme structure modeling of the Aspergillus oryzae N74 fructosyltransferase.Molecular biology reports · 2011Article
- The probiotic Lactobacillus johnsonii NCC 533 produces high-molecular-mass inulin from sucrose by using an inulosucrase enzyme.Applied and environmental microbiology · 2008Article
- Functional role of the additional domains in inulosucrase (IslA) from Leuconostoc citreum CW28.BMC biochemistry · 2008Article
- Structure-function relationships of glucansucrase and fructansucrase enzymes from lactic acid bacteria.Microbiology and molecular biology reviews : MMBR · 2006Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gene coding for inulosucrase in Leuconostoc citreum CW28, islA, was cloned, sequenced, and expressed in Escherichia coli. The recombinant enzyme catalyzed inulin synthesis from sucrose like the wild-type enzyme. Inulosucrase presents an unusual structure: its N-terminal region is similar to the variable region of glucosyltransferases, its catalytic domain is similar to fructosyltransferases from various microorganisms, and its C-terminal domain presents similarity to the glucan binding domain from alternansucrase, a glucosyltransferase from Leuconostoc mesenteroides NRRL B-1355. From sequence comparison, it was found that this fructosyltransferase is a natural chimeric enzyme resulting from the substitution of the catalytic domain of alternansucrase by a fructosyltransferase. Two different forms of the islA gene truncated in the C-terminal glucan binding domain were successfully expressed in E. coli and retained their ability to synthesize inulin but lost thermal stability. This is the first report of an inulosucrase bearing structural features of both glucosyltransferases and fructosyltransferases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.