ArticleJournal of bacteriology2003
Protein synthesis in Escherichia coli with mischarged tRNA.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Genetically stable kill-switch using "demon and angel" expression construct of essential genes.Frontiers in bioengineering and biotechnology · 2024Article
- Impact of queuosine modification of endogenousFrontiers in molecular biosciences · 2022Article
- An Unnatural Amino Acid-Regulated Growth Controller Based on Informational Disturbance.Life (Basel, Switzerland) · 2021Article
- Review
- tRNA acceptor stem and anticodon bases form independent codes related to protein folding.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- The predatory bacterium Bdellovibrio bacteriovorus aspartyl-tRNA synthetase recognizes tRNAAsn as a substrate.PloS one · 2014Article
- Characterization of Gtf1p, the connector subunit of yeast mitochondrial tRNA-dependent amidotransferase.The Journal of biological chemistry · 2011Article
- Misacylation of specific nonmethionyl tRNAs by a bacterial methionyl-tRNA synthetase.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Severe oxidative stress induces protein mistranslation through impairment of an aminoacyl-tRNA synthetase editing site.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Quality control despite mistranslation caused by an ambiguous genetic code.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Conserved discrimination against misacylated tRNAs by two mesophilic elongation factor Tu orthologs.Biochemistry · 2008Article
- Amino acid modifications on tRNA.Acta biochimica et biophysica Sinica · 2008Review
- Aminoacyl-tRNA synthetase complexes: molecular multitasking revealed.FEMS microbiology reviews · 2008Review
- From one amino acid to another: tRNA-dependent amino acid biosynthesis.Nucleic acids research · 2008Review
- Structural elements defining elongation factor Tu mediated suppression of codon ambiguity.Nucleic acids research · 2007Article
- Pyrrolysine analogues as substrates for pyrrolysyl-tRNA synthetase.FEBS letters · 2006Article
- The nondiscriminating aspartyl-tRNA synthetase from Helicobacter pylori: anticodon-binding domain mutations that impact tRNA specificity and heterologous toxicity.Biochemistry · 2006Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two types of aspartyl-tRNA synthetase exist: the discriminating enzyme (D-AspRS) forms only Asp-tRNA(Asp), while the nondiscriminating one (ND-AspRS) also synthesizes Asp-tRNA(Asn), a required intermediate in protein synthesis in many organisms (but not in Escherichia coli). On the basis of the E. coli trpA34 missense mutant transformed with heterologous ND-aspS genes, we developed a system with which to measure the in vivo formation of Asp-tRNA(Asn) and its acceptance by elongation factor EF-Tu. While large amounts of Asp-tRNA(Asn) are detrimental to E. coli, smaller amounts support protein synthesis and allow the formation of up to 38% of the wild-type level of missense-suppressed tryptophan synthetase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.