ArticleMolecular and cellular biology2003
Xkid is degraded in a D-box, KEN-box, and A-box-independent pathway.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 70 citations in OpenAlex.
- STYK1/NOK affects cell cycle late mitosis and directly interacts with anaphase-promoting complex activator CDH1.Heliyon · 2022Article
- Ubiquitination in Plant Meiosis: Recent Advances and High Throughput Methods.Frontiers in plant science · 2021Review
- Mechanisms for the temporal regulation of substrate ubiquitination by the anaphase-promoting complex/cyclosome.Cell division · 2019Review
- Building a Regulatory Network with Short Linear Sequence Motifs: Lessons from the Degrons of the Anaphase-Promoting Complex.Molecular cell · 2016Review
- APC/C and retinoblastoma interaction: cross-talk of retinoblastoma protein with the ubiquitin proteasome pathway.Bioscience reports · 2016Review
- Substrate Recognition by the Cdh1 Destruction Box Receptor Is a General Requirement for APC/CCdh1-mediated Proteolysis.The Journal of biological chemistry · 2016Article
- Targeting Cdc20 as a novel cancer therapeutic strategy.Pharmacology & therapeutics · 2015Review
- The Non-Canonical Role of Aurora-A in DNA Replication.Frontiers in oncology · 2015Review
- Co-activator independent differences in how the metaphase and anaphase APC/C recognise the same substrate.Biology open · 2014Article
- Functional characterization of Anaphase Promoting Complex/Cyclosome (APC/C) E3 ubiquitin ligases in tumorigenesis.Biochimica et biophysica acta · 2014Review
- Insights into degron recognition by APC/C coactivators from the structure of an Acm1-Cdh1 complex.Molecular cell · 2013Article
- The phosphatase-transcription activator EYA1 is targeted by anaphase-promoting complex/Cdh1 for degradation at M-to-G1 transition.Molecular and cellular biology · 2013Article
- REV7 is required for anaphase-promoting complex-dependent ubiquitination and degradation of translesion DNA polymerase REV1.Cell cycle (Georgetown, Tex.) · 2013Article
- Regulation of APC/C-Cdh1 and its function in neuronal survival.Molecular neurobiology · 2012Review
- GPS-ARM: computational analysis of the APC/C recognition motif by predicting D-boxes and KEN-boxes.PloS one · 2012Article
- Structural insights into anaphase-promoting complex function and mechanism.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2011Review
- Arabidopsis ULTRAVIOLET-B-INSENSITIVE4 maintains cell division activity by temporal inhibition of the anaphase-promoting complex/cyclosome.The Plant cell · 2011Article
- Regulation of mitochondrial morphology by APC/CCdh1-mediated control of Drp1 stability.Molecular biology of the cell · 2011Article
- Ama1p-activated anaphase-promoting complex regulates the destruction of Cdc20p during meiosis II.Molecular biology of the cell · 2011Article
- Substrate-specific regulation of ubiquitination by the anaphase-promoting complex.Cell cycle (Georgetown, Tex.) · 2011Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During mitosis, the Xenopus chromokinesin Kid (Xkid) provides the polar ejection forces needed at metaphase for chromosome congression, and its degradation is required at anaphase to induce chromosome segregation. Despite the fact that the degradation of Xkid at anaphase seems to be a key regulatory factor to induce chromosome movement to the poles, little is known about the mechanisms controlling this proteolysis. We investigated here the degradation pathway of Xkid. We demonstrate that Xkid is degraded both in vitro and in vivo by APC/Cdc20 and APC/Cdh1. We show that, despite the presence of five putative D-box motifs in its sequence, Xkid is proteolyzed in a D-box-independent manner. We identify a domain within the C terminus of this chromokinesin, with sequence GxEN, whose mutation completely stabilizes this protein by both APC/Cdc20 and APC/Cdh1. Moreover, we show that this degradation sequence acts as a transposable motif and induces the proteolysis of a GST-GXEN fusion protein. Finally, we demonstrate that both a D-box and a GXEN-containing peptides completely block APC-dependent degradation of cyclin B and Xkid, indicating that the GXEN domain might mediate the recognition and association of Xkid with the APC.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.