ArticleMolecular and cellular biology2003
Cotranscriptional processing of Drosophila histone mRNAs.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Cell-cycle-regulated transcriptional pausing ofMolecular biology of the cell · 2025Article
- The 3' Pol II pausing at replication-dependent histone genes is regulated by Mediator through Cajal bodies' association with histone locus bodies.Nature communications · 2022Article
- Superresolution light microscopy of theMolecular biology of the cell · 2021Article
- Coordinating cell cycle-regulated histone gene expression through assembly and function of the Histone Locus Body.RNA biology · 2017Review
- Concentrating pre-mRNA processing factors in the histone locus body facilitates efficient histone mRNA biogenesis.The Journal of cell biology · 2016Article
- FUS/TLS contributes to replication-dependent histone gene expression by interaction with U7 snRNPs and histone-specific transcription factors.Nucleic acids research · 2015Article
- In vivo characterization of the Drosophila mRNA 3' end processing core cleavage complex.RNA (New York, N.Y.) · 2015Article
- RNA polymerase II transcription elongation control.Chemical reviews · 2013Review
- Functional association of Gdown1 with RNA polymerase II poised on human genes.Molecular cell · 2012Article
- Molecular mechanisms of eukaryotic pre-mRNA 3' end processing regulation.Nucleic acids research · 2010Review
- Isolation and functional analysis of RNA polymerase II elongation complexes.Methods (San Diego, Calif.) · 2009Article
- A core complex of CPSF73, CPSF100, and Symplekin may form two different cleavage factors for processing of poly(A) and histone mRNAs.Molecular cell · 2009Article
- Deadenylation is a widespread effect of miRNA regulation.RNA (New York, N.Y.) · 2009Article
- 3' end mRNA processing: molecular mechanisms and implications for health and disease.The EMBO journal · 2008Review
- Review
- A conserved role for cytoplasmic poly(A)-binding protein 1 (PABPC1) in nonsense-mediated mRNA decay.The EMBO journal · 2007Article
- Review
- U7 snRNA mutations in Drosophila block histone pre-mRNA processing and disrupt oogenesis.RNA (New York, N.Y.) · 2006Article
- Differences and similarities between Drosophila and mammalian 3' end processing of histone pre-mRNAs.RNA (New York, N.Y.) · 2005Article
- 3' Processing of Animal Replication-Dependent Histone mRNAs.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The 3' ends of metazoan histone mRNAs are generated by specialized processing machinery that cleaves downstream of a conserved stem-loop structure. To examine how this reaction might be influenced by transcription, we used a Drosophila melanogaster in vitro system that supports both processes. In this system the complete synthesis of histone mRNA, including transcription initiation and elongation, followed by 3' end formation, occurred at a physiologically significant rate. Processing of free transcripts was efficient and occurred with a t(1/2) of less than 1 min. Divalent cations were not required, but nucleoside triphosphates (NTPs) stimulated the rate of cleavage slightly. Isolated elongation complexes encountered a strong arrest site downstream of the mature histone H4 3' end. In the presence of NTPs, transcripts in these arrested complexes were processed at a rate similar to that of free RNA. Removal of NTPs dramatically reduced this rate, potentially due to concealment of the U7 snRNP binding element. The arrest site was found to be a conserved feature located 32 to 35 nucleotides downstream of the processing site on the H4, H2b, and H3 genes. The significance of the newly discovered arrest sites to our understanding of the coupling between transcription and RNA processing on the one hand and histone gene expression on the other is discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.