ArticleNucleic acids research2003
Genotyping single nucleotide polymorphisms directly from genomic DNA by invasive cleavage reaction on microspheres.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 39 citations in OpenAlex.
- Single Nucleotide Polymorphisms inDiagnostics (Basel, Switzerland) · 2022Review
- Single-nucleotide polymorphism (SNP) genotyping using cationic conjugated polymers in homogeneous solution.Nature protocols · 2009Article
- Single-tube-genotyping of gastric cancer related SNPs by directly using whole blood and paper-dried blood as starting material.World journal of gastroenterology · 2006Article
- Multiplexed and microparticle-based analyses: quantitative tools for the large-scale analysis of biological systems.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2006Review
- The Invader assay for SNP genotyping.Mutation research · 2005Review
- MARA: a novel approach for highly multiplexed locus-specific SNP genotyping using high-density DNA oligonucleotide arrays.Nucleic acids research · 2004Article
- Straightforward detection of SNPs in double-stranded DNA by using exonuclease III/nuclease S1/PNA system.Nucleic acids research · 2004Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we report proof-of-principle for a microsphere-based genotyping assay that detects single nucleotide polymorphisms (SNPs) directly from human genomic DNA samples. This assay is based on a structure-specific cleavage reaction that achieves single base discrimination with a 5'-nuclease which recognizes a tripartite substrate formed upon hybridization of target DNA with probe and upstream oligonucleotides. The assay is simple with two easy steps: a cleavage reaction, which generates fluorescent signal on microsphere surfaces, followed by flow cytometry analysis of the microspheres. Genomic DNA samples were genotyped for the SNP in the Apolipoprotein E gene at amino acid position 158. The assay successfully scored wild type, heterozygous and homozygous mutants. To our knowledge, this is the first report of a solid-support assay for detection of SNPs directly from genomic DNA without PCR amplification of the target.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.