ArticleNucleic acids research2003
In vitro analysis of nuclear mRNA export using molecular beacons for target detection.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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Who cites it
10 citing papers in PubMed, 19 citations in OpenAlex.
- Nucleocytoplasmic shuttling of Gle1 impacts DDX1 at transcription termination sites.Molecular biology of the cell · 2020Article
- Single molecule studies of nucleocytoplasmic transport.Biochimica et biophysica acta · 2011Review
- Molecular beacons: powerful tools for imaging RNA in living cells.Journal of nucleic acids · 2011Article
- Molecular engineering of DNA: molecular beacons.Angewandte Chemie (International ed. in English) · 2009Review
- Molecular beacons in biomedical detection and clinical diagnosis.International journal of clinical and experimental pathology · 2008Article
- Intracellular transport of hepatitis B virus.World journal of gastroenterology · 2007Review
- Superior structure stability and selectivity of hairpin nucleic acid probes with an L-DNA stem.Nucleic acids research · 2007Article
- Dynamics of filamentous viral RNPs prior to egress.Nucleic acids research · 2007Article
- In vivo BiFC analysis of Y14 and NXF1 mRNA export complexes: preferential localization within and around SC35 domains.The Journal of cell biology · 2006Article
- Dynamics of transcription and mRNA export.Current opinion in cell biology · 2005Review
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A detailed molecular characterization of nuclear mRNA export will require an in vitro system, allowing a biochemical reconstitution of transport. To this end, an mRNA export assay has been developed using digitonin-permeabilized HeLa cells and 2'-O-methyl oligoribonucleotide molecular beacons for target detection. These probes allow the homogeneous detection of poly(A)+ RNA at subnanomolar concentrations in the presence of cytosol, without the need for RNA purification and time-consuming methods like northern blotting or RT-PCR. Nuclear export of endogenous mRNA in permeabilized cells occurs in a time- and temperature-dependent manner and can be inhibited by wheat germ agglutinin, indicative of specific transport through nuclear pore complexes. Nuclear export in vitro is insensitive to the depletion of ATP and does not depend on the addition of cytosolic factors, suggesting that shuttling proteins are not required for efficient transport. This is the first demonstration of molecular beacons as powerful tools for the analysis of nucleocytoplasmic RNA transport.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.