ArticleNucleic acids research2003
A novel helper phage that improves phage display selection efficiency by preventing the amplification of phages without recombinant protein.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 56 citations in OpenAlex.
- Construction and characterization of a novel miniaturized filamentous phagemid for targeted mammalian gene transfer.Microbial cell factories · 2023Article
- A Novel Synthetic Antibody Library with Complementarity-Determining Region Diversities Designed for an Improved Amplification Profile.International journal of molecular sciences · 2022Article
- Review
- Open reading frame mining identifies a TLR4 binding domain in the primary sequence of ECRG4.Cellular and molecular life sciences : CMLS · 2019Article
- Universal protection against influenza infection by a multidomain antibody to influenza hemagglutinin.Science (New York, N.Y.) · 2018Article
- A Sortase A Programmable Phage Display Format for Improved Panning of Fab Antibody Libraries.Journal of molecular biology · 2018Article
- Enhancement of therapeutic potential of a naturally occurring human antibody targeting a phosphorylated SerActa neuropathologica communications · 2018Article
- A common antigenic motif recognized by naturally occurring human VActa neuropathologica communications · 2018Article
- A gold nanoparticle-single-chain fragment variable antibody as an immunoprobe for rapid detection of morphine by dipstick.RSC advances · 2018Article
- Article
- Naïve Human Antibody Libraries for Infectious Diseases.Advances in experimental medicine and biology · 2017Review
- Simvastatin Sodium Salt and Fluvastatin Interact with Human Gap Junction Gamma-3 Protein.PloS one · 2016Article
- An improved single-chain Fab platform for efficient display and recombinant expression.Journal of molecular biology · 2015Article
- Bacteriophage vehicles for phage display: biology, mechanism, and application.Current microbiology · 2014Review
- Applying the ResFinder and VirulenceFinder web-services for easy identification of acquired antibiotic resistance andBacteriophage · 2014Article
- Highly conserved protective epitopes on influenza B viruses.Science (New York, N.Y.) · 2012Article
- DeltaPhage--a novel helper phage for high-valence pIX phagemid display.Nucleic acids research · 2012Article
- High affinity, developability and functional size: the holy grail of combinatorial antibody library generation.Molecules (Basel, Switzerland) · 2011Review
- Design and screening of M13 phage display cDNA libraries.Molecules (Basel, Switzerland) · 2011Review
- Progress in phage display: evolution of the technique and its application.Cellular and molecular life sciences : CMLS · 2010Review
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phage display is a widely used technology for the isolation of peptides and proteins with specific binding properties from large libraries of these molecules. A drawback of the common phagemid/helper phage systems is the high infective background of phages that do not display the protein of interest, but are propagated due to non-specific binding to selection targets. This and the enhanced growth rates of bacteria harboring aberrant phagemids not expressing recombinant proteins leads to a serious decrease in selection efficiency. Here we describe a VCSM13-derived helper phage that circumvents this problem, because it lacks the genetic information for the infectivity domains of phage coat protein pIII. Rescue of a library with this novel CT helper phage yields phages that are only infectious when they contain a phagemid-encoded pIII-fusion protein, since phages without a displayed protein carry truncated pIII only and are lost upon re-infection. Importantly, the CT helper phage can be produced in quantities similar to the VCSM13 helper phage. The superiority of CT over VCSM13 during selection was demonstrated by a higher percentage of positive clones isolated from an antibody library after two selection rounds on a complex cellular target. We conclude that the CT helper phage considerably improves the efficiency of selections using phagemid-based protein libraries.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.