ArticleNucleic acids research2003
The role of intercalating residues in chromosomal high-mobility-group protein DNA binding, bending and specificity.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 68 citations in OpenAlex.
- Counteraction of HMGB1 at ss-dsDNA junctions maintains liquidity of protamine-DNA co-condensates.Nature communications · 2026Article
- Counteraction of HMGB1 at ss-dsDNA junctions maintains liquidity of protamine-DNA co-condensates.bioRxiv : the preprint server for biology · 2026Article
- Complexes of HMO1 with DNA: Structure and Affinity.Biomolecules · 2024Article
- Engineering miniature CRISPR-Cas Un1Cas12f1 for efficient base editing.Molecular therapy. Nucleic acids · 2024Article
- Structure and Functions of HMGB2 Protein.International journal of molecular sciences · 2023Review
- Kinetics of DNA looping by Anabaena sensory rhodopsin transducer (ASRT) by using DNA cyclization assay.Scientific reports · 2021Article
- Intercalation of small molecules into DNA in chromatin is primarily controlled by superhelical constraint.PloS one · 2019Article
- Single-molecule studies of high-mobility group B architectural DNA bending proteins.Biophysical reviews · 2017Review
- Two high-mobility group box domains act together to underwind and kink DNA.Acta crystallographica. Section D, Biological crystallography · 2015Article
- Structural and functional conservation of fungal MatA and human SRY sex-determining proteins.Nature communications · 2014Article
- Model of a DNA-protein complex of the architectural monomeric protein MC1 from Euryarchaea.PloS one · 2014Article
- Single-molecule kinetics reveal microscopic mechanism by which High-Mobility Group B proteins alter DNA flexibility.Nucleic acids research · 2013Article
- Transcriptional activation by mitochondrial transcription factor A involves preferential distortion of promoter DNA.Nucleic acids research · 2012Article
- Human mitochondrial transcription factor A induces a U-turn structure in the light strand promoter.Nature structural & molecular biology · 2011Article
- Crystal structure of QscR, a Pseudomonas aeruginosa quorum sensing signal receptor.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Understanding apparent DNA flexibility enhancement by HU and HMGB architectural proteins.Journal of molecular biology · 2011Article
- Structural analysis of HMGD-DNA complexes reveals influence of intercalation on sequence selectivity and DNA bending.Journal of molecular biology · 2010Article
- Biophysical characterization of DNA binding from single molecule force measurements.Physics of life reviews · 2010Review
- The yeast high mobility group protein HMO2, a subunit of the chromatin-remodeling complex INO80, binds DNA ends.Nucleic acids research · 2009Article
- Structural analysis and DNA binding of the HMG domains of the human mitochondrial transcription factor A.Nucleic acids research · 2009Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Ubiquitous high-mobility-group (HMGB) chromosomal proteins bind DNA in a non-sequence- specific fashion to promote chromatin function and gene regulation. Minor groove DNA binding of the HMG domain induces substantial DNA bending toward the major groove, and several interfacial residues contribute by DNA intercalation. The role of the intercalating residues in DNA binding, bending and specificity was systematically examined for a series of mutant Drosophila HMGB (HMG-D) proteins. The primary intercalating residue of HMG-D, Met13, is required both for high-affinity DNA binding and normal DNA bending. Leu9 and Tyr12 directly interact with Met13 and are required for HMG domain stability in addition to linear DNA binding and bending, which is an important function for these residues. In contrast, DNA binding and bending is retained in truncations of intercalating residues Val32 and Thr33 to alanine, but DNA bending is decreased for the glycine substitutions. Furthermore, substitution of the intercalating residues with those predicted to be involved in the specificity of the HMG domain transcription factors results in increased DNA affinity and decreased DNA bending without increased specificity. These studies reveal the importance of residues that buttress intercalating residues and suggest that features of the HMG domain other than a few base-specific hydrogen bonds distinguish the sequence-specific and non-sequence-specific HMG domain functions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.