ArticleThe Journal of cell biology2003
Kinetic analysis of receptor-activated phosphoinositide turnover.
Article in The Journal of cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers.
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Who cites it
105 citing papers in PubMed, 194 citations in OpenAlex.
- From lipid dynamics to precision predictions: A new approach methodology for precision modeling of phosphoinositide signaling.Journal of precision medicine (Amsterdam, Netherlands) · 2026Article
- Single-molecule lipid biosensors mitigate inhibition of endogenous effector proteins.The Journal of cell biology · 2025Article
- Single molecule Lipid Biosensors Mitigate Inhibition of Endogenous Effector Proteins.bioRxiv : the preprint server for biology · 2025Article
- Beyond analytic solution: Analysis of FRAP experiments by spatial simulation of the forward problem.Biophysical journal · 2023Article
- Nanoscale Phosphoinositide Distribution on Cell Membranes of Mouse Cerebellar Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Article
- PI(4,5)P2: signaling the plasma membrane.The Biochemical journal · 2022Article
- Biophysical physiology of phosphoinositide rapid dynamics and regulation in living cells.The Journal of general physiology · 2022Article
- Design principles of PI(4,5)PProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Expression of the GFP-mammalian pleckstrin homology (PH) domain of the phospholipase C δ1 in Saccharomyces cerevisiae BY4741.Molecular biology reports · 2022Article
- Hippocampal neurons maintain a large PtdIns(4)P pool that results in faster PtdIns(4,5)P2 synthesis.The Journal of general physiology · 2022Article
- Quantitative FRET Microscopy Reveals a Crucial Role of Cytoskeleton in Promoting PI(4,5)PInternational journal of molecular sciences · 2021Article
- Loss of diacylglycerol kinase ε causes thrombotic microangiopathy by impairing endothelial VEGFA signaling.JCI insight · 2021Article
- Ten steps to investigate a cellular system with mathematical modeling.PLoS computational biology · 2021Article
- Methods to study phosphoinositide regulation of ion channels.Methods in enzymology · 2021Article
- Control of Neuronal Excitability by Cell Surface Receptor Density and Phosphoinositide Metabolism.Frontiers in pharmacology · 2021Article
- Phosphatidylinositol 4,5-bisphosphate is regenerated by speeding of the PI 4-kinase pathway during long PLC activation.The Journal of general physiology · 2020Article
- ENaC regulation by phospholipids and DGK explained through mathematical modeling.Scientific reports · 2020Article
- A model of the PI cycle reveals the regulating roles of lipid-binding proteins and pitfalls of using mosaic biological data.Scientific reports · 2020Article
- Defining the subcellular distribution and metabolic channeling of phosphatidylinositol.The Journal of cell biology · 2020Article
45 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We studied the bradykinin-induced changes in phosphoinositide composition of N1E-115 neuroblastoma cells using a combination of biochemistry, microscope imaging, and mathematical modeling. Phosphatidylinositol-4,5-bisphosphate (PIP2) decreased over the first 30 s, and then recovered over the following 2-3 min. However, the rate and amount of inositol-1,4,5-trisphosphate (InsP3) production were much greater than the rate or amount of PIP2 decline. A mathematical model of phosphoinositide turnover based on this data predicted that PIP2 synthesis is also stimulated by bradykinin, causing an early transient increase in its concentration. This was subsequently confirmed experimentally. Then, we used single-cell microscopy to further examine phosphoinositide turnover by following the translocation of the pleckstrin homology domain of PLCdelta1 fused to green fluorescent protein (PH-GFP). The observed time course could be simulated by incorporating binding of PIP2 and InsP3 to PH-GFP into the model that had been used to analyze the biochemistry. Furthermore, this analysis could help to resolve a controversy over whether the translocation of PH-GFP from membrane to cytosol is due to a decrease in PIP2 on the membrane or an increase in InsP3 in cytosol; by computationally clamping the concentrations of each of these compounds, the model shows how both contribute to the dynamics of probe translocation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.