ArticleBiophysical journal2003
Nonresonant confocal Raman imaging of DNA and protein distribution in apoptotic cells.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
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Who cites it
84 citing papers in PubMed, 342 citations in OpenAlex.
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- Unveiling the Molecular Secrets: A Comprehensive Review of Raman Spectroscopy in Biological Research.ACS omega · 2024Review
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- Multimodal Molecular Imaging Reveals a Novel Membrane Component in Sporangia of the Rare ActinomyceteACS omega · 2024Article
- Recent Advances in Enhancement of Raman Scattering Intensity for Biological Applications.Chemical & biomedical imaging · 2023Review
- Simultaneous Imaging and Characterization of Polyunsaturated Fatty Acids, Carotenoids, and Microcrystalline Guanine in SingleThe journal of physical chemistry. B · 2023Article
- Video-rate Raman-based metabolic imaging by Airy light-sheet illumination and photon-sparse detection.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Direct imaging of intracellular RNA, DNA, and liquid-liquid phase separated membraneless organelles with Raman microspectroscopy.Communications biology · 2022Article
- Raman Spectroscopy for Chemical Biology Research.Journal of the American Chemical Society · 2022Review
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- Raman Micro-spectroscopy and Imaging of Filamentous Fungi.Microbes and environments · 2022Review
- A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems.RSC chemical biology · 2021Review
- Unveiling osteosarcoma responses to DAPT combined with cisplatin by using confocal Raman microscopy.Biomedical optics express · 2021Article
- Temporal Imaging of Live Cells by High-Speed Confocal Raman Microscopy.Materials (Basel, Switzerland) · 2021Article
- Deuterium-labeled Raman tracking of glucose accumulation and protein metabolic dynamics in Aspergillus nidulans hyphal tips.Scientific reports · 2021Article
- Abiotic stressors impact outer membrane vesicle composition in a beneficial rhizobacterium: Raman spectroscopy characterization.Scientific reports · 2020Article
24 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nonresonant confocal Raman imaging has been used to map the DNA and the protein distributions in individual single human cells. The images are obtained on an improved homebuilt confocal Raman microscope. After statistical analysis, using singular value decomposition, the Raman images are reconstructed from the spectra covering the fingerprint region. The data are obtained at a step interval of approximately 250 nm and cover a field from 8- to 15- micro m square in size. Dwell times at each pixel are between 0.5 and 2 s, depending on the nature and the state of the cell under investigation. High quality nonresonant Raman images can only be obtained under these conditions using continuous wave high laser powers between 60 and 120 mW. We will present evidence that these laser powers can still safely be used to recover the chemical distributions in fixed cells. The developed Raman imaging method is used to image directly, i.e., without prior labeling, the nucleotide condensation and the protein distribution in the so-called nuclear fragments of apoptotic HeLa cells. In the control (nonapoptotic) HeLa cells, we show, for the first time by Raman microspectroscopy, the presence of the RNA in a cell nucleus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.