ArticleBiophysical journal2003
Mechanisms of microtubule-based kinetochore positioning in the yeast metaphase spindle.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
57 citing papers in PubMed, 104 citations in OpenAlex.
- Both sides now: modeling motor regulation of microtubule length at both ends.Physical biology · 2026Article
- Misregulation of cell cycle-dependent methylation of budding yeast CENP-A contributes to chromosomal instability.Molecular biology of the cell · 2023Article
- The microtubule plus-end tracking protein Bik1 is required for chromosome congression.Molecular biology of the cell · 2022Article
- Kre28-Spc105 interaction is essential for Spc105 loading at the kinetochore.Open biology · 2022Article
- Mitotic spindle: lessons from theoretical modeling.Molecular biology of the cell · 2021Article
- Microtubule dynamics regulation reconstituted in budding yeast lysates.Journal of cell science · 2018Article
- Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability.Molecular biology of the cell · 2018Article
- Stu2 acts as a microtubule destabilizer in metaphase budding yeast spindles.Molecular biology of the cell · 2018Article
- Kinesin-5 mediated chromosome congression in insect spindles.Cellular and molecular bioengineering · 2018Article
- Stochastic Modeling Yields a Mechanistic Framework for Spindle Attachment Error Correction in Budding Yeast Mitosis.Cell systems · 2017Article
- Physical determinants of bipolar mitotic spindle assembly and stability in fission yeast.Science advances · 2017Article
- Kinesin-8 effects on mitotic microtubule dynamics contribute to spindle function in fission yeast.Molecular biology of the cell · 2016Article
- Physical limits on kinesin-5-mediated chromosome congression in the smallest mitotic spindles.Molecular biology of the cell · 2015Article
- The kinetochore encodes a mechanical switch to disrupt spindle assembly checkpoint signalling.Nature cell biology · 2015Article
- Cell size modulates oscillation, positioning and length of mitotic spindles.Scientific reports · 2015Article
- The SUMO deconjugating peptidase Smt4 contributes to the mechanism required for transition from sister chromatid arm cohesion to sister chromatid pericentromere separation.Cell cycle (Georgetown, Tex.) · 2015Article
- Assembling the protein architecture of the budding yeast kinetochore-microtubule attachment using FRET.Current biology : CB · 2014Article
- Pericentromere tension is self-regulated by spindle structure in metaphase.The Journal of cell biology · 2014Article
- Bending the rules: widefield microscopy and the Abbe limit of resolution.Journal of cellular physiology · 2014Review
- Budding yeast kinetochore proteins, Chl4 and Ctf19, are required to maintain SPB-centromere proximity during G1 and late anaphase.PloS one · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
It has been hypothesized that spatial gradients in kMT dynamic instability facilitate mitotic spindle formation and chromosome movement. To test this hypothesis requires the analysis of kMT dynamics, which have not been resolved at the single kMT level in living cells. The budding yeast spindle offers an attractive system in which to study kMT dynamics because, in contrast to animal cells, there is only one kMT per kinetochore. To visualize metaphase kMT plus-end dynamics in yeast, a strain containing a green fluorescent protein fusion to the kinetochore protein, Cse4, was imaged by fluorescence microscopy. Although individual kinetochores were not resolvable, we found that models of kMT dynamics could be evaluated by simulating the stochastic kMT dynamics and then simulating the fluorescence imaging of kMT plus-end-associated kinetochores. Statistical comparison of model-predicted images to experimentally observed images demonstrated that a pure dynamic instability model for kMT dynamics in the yeast metaphase spindle was unacceptable. However, when a temporally stable spatial gradient in the catastrophe or rescue frequency was added to the model, there was reasonable agreement between the model and the experiment. These results provide the first evidence of temporally stable spatial gradients of kMT catastrophe and/or rescue frequency in living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.