ArticleMolecular genetics and genomics : MGG2003
Differential modulation of Bordetella pertussis virulence genes as evidenced by DNA microarray analysis.
Article in Molecular genetics and genomics : MGG, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 66 citations in OpenAlex.
- Architecture and regulatory functions of c-di-GMP signaling in classical Bordetella species.FEMS microbiology reviews · 2026Review
- Prevalence of Pertactin-Deficient Bordetella pertussis Isolates, Slovenia.Emerging infectious diseases · 2024Article
- Phosphorylation chemistry of the Bordetella PlrSR TCS and its contribution to bacterial persistence in the lower respiratory tract.Molecular microbiology · 2023Article
- Deep longitudinal multi-omics analysis ofFrontiers in microbiology · 2023Article
- Nanomotion Spectroscopy as a New Approach to Characterize Bacterial Virulence.Microorganisms · 2021Article
- Review
- Analysis of themSphere · 2019Article
- A Novel Bvg-Repressed Promoter CausesJournal of bacteriology · 2018Article
- Comparison of the Whole Cell Proteome and Secretome of EpidemicFrontiers in microbiology · 2018Article
- Primary transcriptome analysis reveals importance of IS elements for the shaping of the transcriptional landscape of Bordetella pertussis.RNA biology · 2018Article
- Activation of Bvg-Repressed Genes in Bordetella pertussis by RisA Requires Cross Talk from Noncooperonic Histidine Kinase RisK.Journal of bacteriology · 2017Article
- The BvgAS Regulon ofmBio · 2017Article
- Article
- Pertussis: Microbiology, Disease, Treatment, and Prevention.Clinical microbiology reviews · 2016Review
- Transcriptional profiling of Bordetella pertussis reveals requirement of RNA chaperone Hfq for Type III secretion system functionality.RNA biology · 2015Article
- Pertussis toxin improves immune responses to a combined pneumococcal antigen and leads to enhanced protection against Streptococcus pneumoniae.Clinical and vaccine immunology : CVI · 2014Article
- Bordetella pertussis pathogenesis: current and future challenges.Nature reviews. Microbiology · 2014Review
- Evidence for a role of the polysaccharide capsule transport proteins in pertussis pathogenesis.PloS one · 2014Article
- In vivo phosphorylation dynamics of the Bordetella pertussis virulence-controlling response regulator BvgA.Molecular microbiology · 2013Article
- Laboratory adaptation of Bordetella pertussis is associated with the loss of type three secretion system functionality.Infection and immunity · 2011Article
Corrections and comments
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Authors and funding
18 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The production of most factors involved in Bordetella pertussis virulence is controlled by a two-component regulatory system termed BvgA/S. In the Bvg+ phase virulence-activated genes (vags) are expressed, and virulence-repressed genes (vrgs) are down-regulated. The expression of these genes can also be modulated by MgSO(4) or nicotinic acid. In this study we used microarrays to analyse the influence of BvgA/S or modulation on the expression of nearly 200 selected genes. With the exception of one vrg, all previously known vags and vrgs were correctly assigned as such, and the microarray analyses identified several new vags and vrgs, including genes coding for putative autotransporters, two-component systems, extracellular sigma factors, the adenylate cyclase accessory genes cyaBDE, and two genes coding for components of a type III secretion system. For most of the new vrgs and vags the results of the microarray analyses were confirmed by RT-PCR analysis and/or lacZfusions. The degree of regulation and modulation varied between genes, and showed a continuum from strongly BvgA/S-activated genes to strongly BvgA/S-repressed genes. The microarray analyses also led to the identification of a subset of vags and vrgs that are differentially regulated and modulated by MgSO(4) or nicotinic acid, indicating that these genes may be targets for multiple regulatory circuits. For example, the expression of bilA, a gene predicted to encode an intimin-like protein, was found to be activated by BvgA/S and up-modulated by nicotinic acid. Furthermore, surprisingly, in the strain analysed here, which produces only type 2 fimbriae, the fim3 gene was identified as a vrg, while fim2 was confirmed to be a vag.
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