ArticleJournal of virology2003
Charged assembly helix motif in murine leukemia virus capsid: an important region for virus assembly and particle size determination.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 39 citations in OpenAlex.
- Distinct stabilization of the human T cell leukemia virus type 1 immature Gag lattice.Nature structural & molecular biology · 2025Article
- Structural Determinants of Virion Assembly and Release in the C Terminus of the Mason-Pfizer Monkey Virus Capsid Protein.Journal of virology · 2021Article
- Effect of Small Polyanions on In Vitro Assembly of Selected Members of Alpha-, Beta- and Gammaretroviruses.Viruses · 2021Article
- ER/K-link-Leveraging a native protein linker to probe dynamic cellular interactions.Methods in enzymology · 2021Review
- Maturation of retroviruses.Current opinion in virology · 2019Review
- Structure and architecture of immature and mature murine leukemia virus capsids.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Functional and Structural Characterization of Novel Type of Linker Connecting Capsid and Nucleocapsid Protein Domains in Murine Leukemia Virus.The Journal of biological chemistry · 2016Article
- Ebselen, a Small-Molecule Capsid Inhibitor of HIV-1 Replication.Antimicrobial agents and chemotherapy · 2016Article
- Dimerization of the SP1 Region of HIV-1 Gag Induces a Helical Conformation and Association into Helical Bundles: Implications for Particle Assembly.Journal of virology · 2016Article
- Atomic Modeling of an Immature Retroviral Lattice Using Molecular Dynamics and Mutagenesis.Structure (London, England : 1993) · 2015Article
- Role of Mason-Pfizer monkey virus CA-NC spacer peptide-like domain in assembly of immature particles.Journal of virology · 2014Article
- Higher-order structure of the Rous sarcoma virus SP assembly domain.Journal of virology · 2014Article
- In vitro assembly of virus-like particles of a gammaretrovirus, the murine leukemia virus XMRV.Journal of virology · 2012Article
- Assembly and architecture of HIV.Advances in experimental medicine and biology · 2012Review
- Solution properties of murine leukemia virus gag protein: differences from HIV-1 gag.Journal of virology · 2011Article
- Design of in vitro symmetric complexes and analysis by hybrid methods reveal mechanisms of HIV capsid assembly.Journal of molecular biology · 2011Article
- On the role of the SP1 domain in HIV-1 particle assembly: a molecular switch?Journal of virology · 2011Article
- The TY3 Gag3 spacer controls intracellular condensation and uncoating.Journal of virology · 2011Article
- Analysis of the N-terminal region of the murine leukemia virus nucleocapsid protein.Virus research · 2011Article
- NMR relaxation studies of an RNA-binding segment of the rous sarcoma virus gag polyprotein in free and bound states: a model for autoinhibition of assembly.Biochemistry · 2010Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
We have identified a region near the C terminus of capsid (CA) of murine leukemia virus (MLV) that contains many charged residues. This motif is conserved in various lengths in most MLV-like viruses. One exception is that spleen necrosis virus (SNV) does not contain a well-defined domain of charged residues. When 33 amino acids of the MLV motif were deleted to mimic SNV CA, the resulting mutant produced drastically reduced amounts of virions and the virions were noninfectious. Furthermore, these viruses had abnormal sizes, often contained punctate structures resembling those in the cell cytoplasm, and packaged both ribosomal and viral RNA. When 11 or 15 amino acids were deleted to modify the MLV CA to resemble those from other gammaretroviruses, the deletion mutants produced virions at levels comparable to those of the wild-type virus and were able to complete one round of virus replication without detectable defects. We generated 10 more mutants that displayed either the wild-type or mutant phenotype. The distribution of the wild-type or mutant phenotype did not directly correlate with the number of amino acids deleted, suggesting that the function of the motif is determined not simply by its length but also by its structure. Structural modeling of the wild-type and mutant proteins suggested that this region forms alpha-helices; thus, we termed this motif the "charged assembly helix." This is the first description of the charged assembly helix motif in MLV CA and demonstration of its role in virus budding and assembly.
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