ArticleJournal of biomolecular NMR2003
A rapid method to attain isotope labeled small soluble peptides for NMR studies.
Article in Journal of biomolecular NMR, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Molecular basis of the biogenesis of a protein organelle for ethanolamine utilization.Science advances · 2025Article
- NMR- and MD simulation-based structural characterization of the membrane-associating FATC domain of ataxia telangiectasia mutated.The Journal of biological chemistry · 2019Article
- The Polycystin-1, Lipoxygenase, and α-Toxin Domain Regulates Polycystin-1 Trafficking.Journal of the American Society of Nephrology : JASN · 2016Article
- The influenza hemagglutinin fusion domain is an amphipathic helical hairpin that functions by inducing membrane curvature.The Journal of biological chemistry · 2015Article
- Recombinant production of the amino terminal cytoplasmic region of dengue virus non-structural protein 4A for structural studies.PloS one · 2014Article
- Analysis of the RelA:CBP/p300 interaction reveals its involvement in NF-κB-driven transcription.PLoS biology · 2013Article
- NMR- and circular dichroism-monitored lipid binding studies suggest a general role for the FATC domain as membrane anchor of phosphatidylinositol 3-kinase-related kinases (PIKK).The Journal of biological chemistry · 2013Article
- Sequence-specific determination of protein and peptide concentrations by absorbance at 205 nm.Protein science : a publication of the Protein Society · 2013Article
- A fast and simple method for probing the interaction of peptides and proteins with lipids and membrane-mimetics using GB1 fusion proteins and NMR spectroscopy.Protein science : a publication of the Protein Society · 2012Article
- Structural basis of RNA binding by leucine zipper GCN4.Protein science : a publication of the Protein Society · 2012Article
- Engineered human antibody constant domains with increased stability.The Journal of biological chemistry · 2009Article
- Dynamics of antibody domains studied by solution NMR.Methods in molecular biology (Clifton, N.J.) · 2009Article
- Mocr: a novel fusion tag for enhancing solubility that is compatible with structural biology applications.Protein expression and purification · 2009Article
- Overexpression of post-translationally modified peptides in Escherichia coli by co-expression with modifying enzymes.Protein expression and purification · 2008Article
- Embryonic neural inducing factor churchill is not a DNA-binding zinc finger protein: solution structure reveals a solvent-exposed beta-sheet and zinc binuclear cluster.Journal of molecular biology · 2007Article
- Rapid preparation of stable isotope labeled peptides that bind to target proteins by a phage library system.Journal of biomolecular NMR · 2006Article
- Protein-Inhibitor Interaction Studies Using NMR.Applications of NMR spectroscopyArticle
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A widely applicable strategy is presented for efficient and rapid production of small water soluble peptides expressed as fusion proteins with the immunoglobulin-binding domain of streptococcal protein G. A simple extraction and purification scheme that includes a protease cleavage step to release the target peptide is described. The yield of authentic target peptide exceeds 10 mg per liter of culture. Production of U-13C, 15N and highly deuterated U-13C, 15N isotope labeled peptide is demonstrated for the 11 residue S2 peptide, corresponding to the C-terminus of the alpha-subunit of transducin, and the coiled coil trimerization domain from cartilage matrix protein (CMPcc), respectively. Heteronuclear two-dimensional NMR spectra are used for initial peptide characterization.
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12766417What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.