ArticleProceedings of the National Academy of Sciences of the United States of America2003
Uniform polarity microtubule assemblies imaged in native brain tissue by second-harmonic generation microscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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Who cites it
77 citing papers in PubMed.
- Screening the optimal rTSMS frequency to orchestrate immune-fibrotic remodeling for adult spinal cord repair.Frontiers in neuroscience · 2026Article
- Tissue type and tissue depth dependency of pulse broadening in three-photon microscopy: impact on third harmonic generation.Biomedical optics express · 2025Article
- Second harmonic generation for brain imaging: pathology-related studies.Biophysical reviews · 2025Article
- Dynamic imaging of myelin pathology in physiologically preserved human brain tissue using third harmonic generation microscopy.PloS one · 2025Article
- Consciousness and spintronic coherence in microtubules.Communicative & integrative biology · 2025Article
- Protective effects of nicotinamide in a mouse model of glaucoma DBA/2 studied by second-harmonic generation microscopy.bioRxiv : the preprint server for biology · 2024Article
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- Differential protection by nicotinamide in a mouse model of glaucoma DBA/2J revealed by second-harmonic generation microscopy.PloS one · 2024Article
- Viewing life without labels under optical microscopes.Communications biology · 2023Review
- Second harmonic generation microscopy: a powerful tool for bio-imaging.Biophysical reviews · 2023Review
- Cellular cartography: Towards an atlas of the neuronal microtubule cytoskeleton.Frontiers in cell and developmental biology · 2023Review
- Axonal architecture of the mouse inner retina revealed by second harmonic generation.PNAS nexus · 2022Article
- H-ABC tubulinopathy revealed by label-free second harmonic generation microscopy.Scientific reports · 2022Article
- Deep learning augmented microscopy: a faster, wider view, higher resolution autofluorescence-harmonic microscopy.Light, science & applications · 2022Article
- Spectroscopic second and third harmonic generation microscopy using a femtosecond laser source in the third near-infrared (NIR-III) optical window.Biomedical optics express · 2022Article
- Advances in nonlinear optical microscopy techniques for in vivo and in vitro neuroimaging.Biophysical reviews · 2021Review
- Stokes polarimetry-based second harmonic generation microscopy for collagen and skeletal muscle fiber characterization.Lasers in medical science · 2021Article
- Article
- Watt W. Webb: His measurements of the seemingly inaccessible broadened the horizons of biophysics.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Harmonic optical tomography of nonlinear structures.Nature photonics · 2020Article
17 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
Abstract
Microtubule (MT) ensemble polarity is a diagnostic determinant of the structure and function of neuronal processes. Here, polarized MT structures are selectively imaged with second-harmonic generation (SHG) microscopy in native brain tissue. This SHG is found to colocalize with axons in both brain slices and cultured neurons. Because SHG arises only from noninversion symmetric structures, the uniform polarity of axonal MTs leads to the observed signal, whereas the mixed polarity in dendrites leads to destructive interference. SHG imaging provides a tool to investigate the kinetics and function of MT ensemble polarity in dynamic native brain tissue structures and other subcellular motility structures based on polarized MTs.
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