ArticleInvestigative ophthalmology & visual science2003
Molecular characterization of the skate peripherin/rds gene: relationship to its orthologues and paralogues.
Article in Investigative ophthalmology & visual science, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Article
- ROM1 is redundant to PRPH2 as a molecular building block of photoreceptor disc rims.bioRxiv : the preprint server for biology · 2023Article
- Structural and functional relationships between photoreceptor tetraspanins and other superfamily members.Cellular and molecular life sciences : CMLS · 2012Review
- The function of oligomerization-incompetent RDS in rods.Advances in experimental medicine and biology · 2010Article
- A partial structural and functional rescue of a retinitis pigmentosa model with compacted DNA nanoparticles.PloS one · 2009Article
- Outer segment oligomerization of Rds: evidence from mouse models and subcellular fractionation.Biochemistry · 2008Article
- Genetic supplementation of RDS alleviates a loss-of-function phenotype in C214S model of retinitis pigmentosa.Advances in experimental medicine and biology · 2008Article
- Late-onset cone photoreceptor degeneration induced by R172W mutation in Rds and partial rescue by gene supplementation.Investigative ophthalmology & visual science · 2007Article
- The Cys214-->Ser mutation in peripherin/rds causes a loss-of-function phenotype in transgenic mice.The Biochemical journal · 2005Article
- Role of the second intradiscal loop of peripherin/rds in homo and hetero associations.Biochemistry · 2005Article
- Modulating expression of peripherin/rds in transgenic mice: critical levels and the effect of overexpression.Investigative ophthalmology & visual science · 2004Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
purposeA great deal of information about functionally significant domains of a protein may be obtained by comparison of primary sequences of gene homologues over a broad phylogenetic base. This study was designed to identify evolutionarily conserved domains of the photoreceptor disc membrane protein peripherin/rds by analysis of the homologue in a primitive vertebrate, the skate.
methodsA skate retinal cDNA library was screened using a mouse peripherin/rds clone. The 5' and 3' untranslated regions of the skate peripherin/rds (srds) cDNA were isolated by the rapid amplification of cDNA ends (RACE) approach. The gene structure was characterized by PCR amplification and sequencing of genomic fragments. Northern and Western blot analyses were used to identify srds transcript and protein, respectively.
resultsA new homologue of peripherin/rds was identified from the skate retinal cDNA library. SRDS is a glycoprotein with a predicted molecular mass of 40.2 kDa. The srds gene consists of two exons and one small intron and transcribes into a single 6-kb message. Phylogenetic analysis places SRDS at the base of peripherin/rds family and near the division of that group and the branch leading to rds-like and rom-1 genes. SRDS protein is 54.5% identical with peripherin/rds across species. Identity is significantly higher (73%) in the intradiscal domains. Sequence comparison revealed the conservation of all residues that have been shown, on mutation, to associate with retinitis pigmentosa and showed conservation of most residues associated with macular dystrophies. Comparison with ROM-1 and other rds-like proteins revealed the presence of a highly conserved domain in the large intradiscal loop.
conclusionsSrds represents the skate orthologue of mammalian peripherin/rds genes. Conservation of most of the residues associated with human retinal diseases indicates that these residues serve important functional roles. The high degree of conservation of a short stretch within the large intradiscal loop also suggests an important function for this domain.
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