ArticleProtein science : a publication of the Protein Society2003
A wheat germ cell-free system is a novel way to screen protein folding and function.
Article in Protein science : a publication of the Protein Society, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed.
- Half a Century of Progress: The Evolution of Wheat Germ-Based In Vitro Translation into a Versatile Protein Production Method.International journal of molecular sciences · 2025Review
- Multiple Gene Expression in Cell-Free Protein Synthesis Systems for Reconstructing Bacteriophages and Metabolic Pathways.Microorganisms · 2022Review
- Cell-free riboswitches.RSC chemical biology · 2021Review
- Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications.Frontiers in molecular biosciences · 2021Article
- Easy Synthesis of Complex Biomolecular Assemblies: Wheat Germ Cell-Free Protein Expression in Structural Biology.Frontiers in molecular biosciences · 2021Review
- Development of a cell-free protein synthesis system for practical use.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2021Article
- Review
- A User's Guide to Cell-Free Protein Synthesis.Methods and protocols · 2019Review
- Exploring the Potential of Cell-Free Protein Synthesis for Extending the Abilities of Biological Systems.Frontiers in bioengineering and biotechnology · 2019Review
- Evaluation of approaches to identify the targets of cellular immunity on a proteome-wide scale.PloS one · 2011Article
- Practical cell-free protein synthesis system using purified wheat embryos.Nature protocols · 2010Article
- An Escherichia coli-based cell-free system for large-scale production of functional mammalian membrane proteins suitable for X-ray crystallography.Journal of molecular microbiology and biotechnology · 2010Article
- NMR-based characterization of a refolding intermediate of beta2-microglobulin labeled using a wheat germ cell-free system.Protein science : a publication of the Protein Society · 2009Article
- Stable-isotope labeling using an inducible viral infection system in suspension-cultured plant cells.Journal of biomolecular NMR · 2008Article
- A novel way of amino acid-specific assignment in (1)H-(15)N HSQC spectra with a wheat germ cell-free protein synthesis system.Journal of biomolecular NMR · 2004Article
- NMR spectroscopic filtration of polypeptides and proteins in complex mixtures.Journal of biomolecular NMR · 2004Article
- In vitro synthesis of fully functional EmrE, a multidrug transporter, and study of its oligomeric state.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Article
- Cell-free protein synthesis: applications come of age.Biotechnology advancesReview
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5 authors.
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Abstract
For high-throughput protein structural analysis, it is indispensable to develop a reliable protein overexpression system. Although many protein overexpression systems, such as that involving Escherichia coli cells, have been developed, the number of overexpressed proteins showing the same biological activities as those of the native proteins is limited. A novel wheat germ cell-free protein synthesis system was developed recently, and most of the proteins functioning in solution were synthesized as soluble forms. This suggests the applicability of this protein synthesis method to determination of the solution structures of functional proteins. To examine this possibility, we have synthesized two (15)N-labeled proteins and obtained (1)H-(15)N HSQC spectra for them. The structural analysis of these proteins has already progressed with an E. coli overexpression system, and (1)H-(15)N HSQC spectra for biologically active proteins have already been obtained. Comparing the spectra, we have shown that proteins synthesized with a wheat germ cell-free system have the proper protein folding and enough biological activity. This is the first experimental evidence of the applicability of the wheat germ cell-free protein synthesis system to high-throughput protein structural analysis.
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