ArticleInfection and immunity2003
Purification and characterization of enterotoxigenic El Tor-like hemolysin produced by Vibrio fluvialis.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 53 citations in OpenAlex.
- Multidrug Resistance, Biofilm-Forming Ability, and Molecular Characterization ofAntibiotics (Basel, Switzerland) · 2025Article
- Haemolysins are essential to the pathogenicity of deep-seaiScience · 2024Article
- Draft-genome analysis provides insights into the virulence properties and genome plasticity of Vibrio fluvialis organisms isolated from shrimp farms and Turag river in Bangladesh.Archives of microbiology · 2022Article
- Population genomics of the food-borne pathogenMicrobial genomics · 2022Article
- Molecular Detection and Distribution of Six Medically ImportantFrontiers in microbiology · 2021Article
- Bacterial Nucleobases Synergistically Induce Larval Settlement and Metamorphosis in the Invasive MusselApplied and environmental microbiology · 2019Article
- Vibrio Pathogens: A Public Health Concern in Rural Water Resources in Sub-Saharan Africa.International journal of environmental research and public health · 2017Review
- Functional Characterization and Conditional Regulation of the Type VI Secretion System inFrontiers in microbiology · 2017Article
- Prevalence and characterisation of non-cholerae Vibrio spp. in final effluents of wastewater treatment facilities in two districts of the Eastern Cape Province of South Africa: implications for public health.Environmental science and pollution research international · 2015Article
- A critical role for hemolysin in Vibrio fluvialis-induced IL-1β secretion mediated by the NLRP3 inflammasome in macrophages.Frontiers in microbiology · 2015Article
- Identification of genetic bases of vibrio fluvialis species-specific biochemical pathways and potential virulence factors by comparative genomic analysis.Applied and environmental microbiology · 2014Article
- Vibrio fluvialis: an emerging human pathogen.Frontiers in microbiology · 2014Review
- Quorum sensing regulatory cascades control Vibrio fluvialis pathogenesis.Journal of bacteriology · 2013Article
- Evidence of a new metabolic capacity in an emerging diarrheal pathogen: lessons from the draft genomes of Vibrio fluvialis strains PG41 and I21563.Gut pathogens · 2013Article
- The virulence phenotypes and molecular epidemiological characteristics of Vibrio fluvialis in China.Gut pathogens · 2013Article
- Seasonal abundance and distribution of Vibrio species in the treated effluent of wastewater treatment facilities in suburban and urban communities of Eastern Cape Province, South Africa.Journal of microbiology (Seoul, Korea) · 2011Article
- Vibrio fluvialis: an unusual enteric pathogen of increasing public health concern.International journal of environmental research and public health · 2010Review
- Laboratory indicators for early detection and surgical treatment of vibrio necrotizing fasciitis.Clinical orthopaedics and related research · 2010Article
- Overexpression, purification, characterization, and pathogenicity of Vibrio harveyi hemolysin VHH.Infection and immunity · 2006Article
- Identification of an iron-regulated hemin-binding outer membrane protein, HupO, in Vibrio fluvialis: effects on hemolytic activity and the oxidative stress response.Infection and immunity · 2005Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The halophilic bacterium Vibrio fluvialis is an enteric pathogen that produces an extracellular hemolysin. This hemolysin was purified to homogeneity by using sequential hydrophobic-interaction chromatography with phenyl-Sepharose CL-4B and gel filtration with Sephacryl S-200. It has a molecular weight of 63,000 and an isoelectric point of 4.6, and its hemolytic activity is sensitive to heat, proteases, and preincubation with zinc ions. The hemolysin lyses erythrocytes of the eight different animal species that we tested, is cytotoxic against Chinese hamster ovary cells in tissue culture, and elicits fluid accumulation in suckling mice. Lysis of erythrocytes occurs by a temperature-dependent binding step followed by a temperature- and pH-dependent lytic step. Fourteen of the first 20 N-terminal amino acid residues (Val-Ser-Gly-Gly-Glu-Ala-Asn-Thr-Leu-Pro-His-Val-Ala-Phe-Tyr-Ile-Asn-Val-Asn-Arg) are identical to those of the El Tor hemolysin of Vibrio cholerae and the heat-labile hemolysin of Vibrio mimicus. This homology was further confirmed by PCR analysis using a 5' primer derived from the amino-terminal sequence of the hemolysin and a 3' primer derived from the El Tor hemolysin structural gene. The hemolysin also reacts with antibodies to the El Tor-like hemolysin of non-O1 V. cholerae.
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