ArticleInfection and immunity2003
Expression of multiple CPB genes encoding cysteine proteases is required for Leishmania mexicana virulence in vivo.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 77 citations in OpenAlex.
- Highlights in Cysteine Protease Inhibitors as Antiparasitic Agents Against Trypanosomatids.International journal of molecular sciences · 2026Review
- LABCG1 AffectsACS infectious diseases · 2026Article
- Development of DNA Aptamers AgainstPharmaceutics · 2026Article
- Evolutionary divergent clusters of transcribed extinct truncated retroposons drive low mRNA expression and developmental regulation in the protozoan Leishmania.BMC biology · 2024Article
- Assessing proteases and enzymes of the trypanothione system in subpopulations of Leishmania (Viannia) braziliensis Thor strain during macrophage infection.Memorias do Instituto Oswaldo Cruz · 2024Article
- Flavonoid Derivatives as New Potent Inhibitors of Cysteine Proteases: An Important Step toward the Design of New Compounds for the Treatment of Leishmaniasis.Microorganisms · 2023Article
- Tissue Specific Dual RNA-Seq Defines Host-Parasite Interplay in Murine Visceral Leishmaniasis Caused by Leishmania donovani and Leishmania infantum.Microbiology spectrum · 2022Article
- Three types ofFrontiers in cellular and infection microbiology · 2022Article
- Detection of Metalloproteases and Cysteine Proteases RNA Transcripts ofBioMed research international · 2020Article
- Cysteine proteases in protozoan parasites.PLoS neglected tropical diseases · 2018Review
- Article
- Cysteine Peptidase B Regulates Leishmania mexicana Virulence through the Modulation of GP63 Expression.PLoS pathogens · 2016Article
- The genetics of Leishmania virulence.Medical microbiology and immunology · 2015Review
- Proteinases as virulence factors in Leishmania spp. infection in mammals.Parasites & vectors · 2012Article
- Leishmania donovani argininosuccinate synthase is an active enzyme associated with parasite pathogenesis.PLoS neglected tropical diseases · 2012Article
- How pathogen-derived cysteine proteases modulate host immune responses.Advances in experimental medicine and biology · 2011Review
- Article
- Overexpression of the natural inhibitor of cysteine peptidases in Leishmania mexicana leads to reduced virulence and a Th1 response.Infection and immunity · 2009Article
- Transgenic Leishmania and the immune response to infection.Parasite immunology · 2008Review
- Fibronectin binding and proteolytic degradation by Leishmania and effects on macrophage activation.Infection and immunity · 2008Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leishmania mexicana mutants deficient in the multicopy CPB gene array have reduced virulence, demonstrated by poor lesion growth in BALB/c mice and induction of a protective Th1 response. Reinsertion of the amastigote-specific CPB2.8 or metacyclic stage-specific CPB2 gene into a CPB-deficient mutant L. mexicana failed to restore either a Th2 response or sustained virulence. However, reexpression of multiple CPB genes from a cosmid significantly restored virulence. This was characterized by increased lesion and parasite growth and the acquisition of a Th2 response, as determined by measuring interleukin-4 production and immunoglobulin G1 (IgG1) and IgE levels. These studies confirm that L. mexicana cysteine proteases are important virulence factors and provide an explanation for the presence in L. mexicana of a multicopy tandem array of CPB genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.