ArticleJournal of clinical immunology2003
Treatment with an anti-CD14 monoclonal antibody delays and inhibits lipopolysaccharide-induced gene expression in humans in vivo.
Article in Journal of clinical immunology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 59 citations in OpenAlex.
- Monoclonal Antibody to CD14, TLR4, or CD11b: Impact of Epitope and Isotype Specificity on ROS Generation by Human Granulocytes and Monocytes.Oxidative medicine and cellular longevity · 2020Review
- Impact of CD14 on Reactive Oxygen Species Production from Human Leukocytes Primed byOxidative medicine and cellular longevity · 2019Article
- CD14 Counterregulates Lipopolysacharide-Induced Tumor Necrosis Factor-α Production in a Macrophage Subset.Journal of innate immunity · 2019Article
- Inhibition of the cluster of differentiation 14 innate immunity pathway with IAXO-101 improves chronic microelectrode performance.Journal of neural engineering · 2018Article
- Dual inhibition of complement and Toll-like receptors as a novel approach to treat inflammatory diseases-C3 or C5 emerge together with CD14 as promising targets.Journal of leukocyte biology · 2017Review
- Protease activated receptor 2 in diabetic nephropathy: a double edged sword.American journal of translational research · 2017Article
- Lgr4/Gpr48 negatively regulates TLR2/4-associated pattern recognition and innate immunity by targeting CD14 expression.The Journal of biological chemistry · 2013Article
- Chitohexaose activates macrophages by alternate pathway through TLR4 and blocks endotoxemia.PLoS pathogens · 2012Article
- Genome-wide expression profiling and mutagenesis studies reveal that lipopolysaccharide responsiveness appears to be absolutely dependent on TLR4 and MD-2 expression and is dependent upon intermolecular ionic interactions.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Rapid screening of innate immune gene expression in zebrafish using reverse transcription - multiplex ligation-dependent probe amplification.BMC research notes · 2011Article
- In vivo endotoxin synchronizes and suppresses clock gene expression in human peripheral blood leukocytes.Critical care medicine · 2010Article
- Expression and function of macrophage migration inhibitory factor (MIF) in melioidosis.PLoS neglected tropical diseases · 2010Article
- A novel model of common Toll-like receptor 4- and injury-induced transcriptional themes in human leukocytes.Critical care (London, England) · 2010Article
- Differential gene expression changes in children with severe dengue virus infections.PLoS neglected tropical diseases · 2008Article
- Effect of acute hyperglycaemia and/or hyperinsulinaemia on proinflammatory gene expression, cytokine production and neutrophil function in humans.Diabetic medicine : a journal of the British Diabetic Association · 2008Article
- Endogenous interleukin-18 improves the early antimicrobial host response in severe melioidosis.Infection and immunity · 2007Article
- Toll-like receptor 2 impairs host defense in gram-negative sepsis caused by Burkholderia pseudomallei (Melioidosis).PLoS medicine · 2007Article
- High-throughput mRNA profiling characterizes the expression of inflammatory molecules in sepsis caused by Burkholderia pseudomallei.Infection and immunity · 2007Article
- Consequence of functional Nod2 and Tlr4 mutations on gene transcription in Crohn's disease patients.Journal of molecular medicine (Berlin, Germany) · 2005Article
- Gene expression profiling of minimal residual disease in acute myeloid leukaemia by novel multiplex-PCR-based method.Leukemia · 2004Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CD14 is a receptor important for activation of cells by lipopolysaccharide (LPS). Treatment with the CD14 antibody IC14 was previously found to attenuate the release of proinflammatory cytokines and some chemokines into the circulation of healthy humans intravenously injected with LPS. To determine the role of circulating leukocytes in CD14-dependent gene expression, 16 healthy volunteers received LPS preceded by either IC14 or placebo. At different time points, mRNA was isolated from whole blood and gene expression was determined by multiplex ligation-dependent probe amplification (MLPA). LPS induced MIP-1alpha, MIP-1beta, IL-8, IL-1beta, and IL-1Ra mRNA production, which was delayed by 1 hr and reduced twofold by IC14 treatment. TNFR1 was unresponsive, whereas other investigated cytokines remained undetectable. Further, LPS showed differential effects on NFkappaB gene expression. LPS induced IkappaBalpha production, whereas p50 was unresponsive and p65 and p49/p100 remained undetectable. LPS induced IkappaBalpha expression was delayed (1 hr) and reduced by IC14. Gene expression profiles in blood cells corresponded poorly with observed changes in plasma levels. These data suggest that peripheral blood cells are of negligible importance in LPS-induced production of inflammatory mediators in vivo and that LPS may activate genes via a CD14-independent pathway that is slower and less efficient.
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