ArticleMolecular genetics and genomics : MGG2003
Genetic evidence for a role of phospholipase C at the budding yeast kinetochore.
Article in Molecular genetics and genomics : MGG, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 21 citations in OpenAlex.
- Phospholipase C: Diverse functions in plant biotic stress resistance and fungal pathogenicity.Molecular plant pathology · 2023Review
- Yeast phospholipase C is required for stability of casein kinase I Yck2p and expression of hexose transporters.FEMS microbiology letters · 2017Article
- Arabidopsis phosphatidylinositol-phospholipase C2 (PLC2) is required for female gametogenesis and embryo development.Planta · 2017Article
- The observation of plcA mutation and localization in Aspergillus nidulans.Journal of microbiology (Seoul, Korea) · 2014Article
- Yeast phospholipase C is required for normal acetyl-CoA homeostasis and global histone acetylation.The Journal of biological chemistry · 2013Article
- Facilitated assembly of the preinitiation complex by separated tail and head/middle modules of the mediator.Journal of molecular biology · 2012Article
- Plc1p is required for proper chromatin structure and activity of the kinetochore in Saccharomyces cerevisiae by facilitating recruitment of the RSC complex.Molecular genetics and genomics : MGG · 2009Article
- Saccharomyces cerevisiae phospholipase C regulates transcription of Msn2p-dependent stress-responsive genes.Eukaryotic cell · 2008Article
- Plc1p is required for SAGA recruitment and derepression of Sko1p-regulated genes.Molecular biology of the cell · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Chromosome segregation during mitosis requires kinetochores, specialized organelles that mediate chromosome attachment to spindle microtubules. We have shown previously that in budding yeast, Plc1p (phosphoinositide-specific phospholipase C) localizes to centromeric loci, associates with the kinetochore proteins Ndc10p and Cep3p, and affects the function of kinetochores. Deletion of PLC1 results in nocodazole sensitivity, mitotic delay, and a higher frequency of chromosome loss. We report here that despite the nocodazole sensitivity of plc1Delta cells, Plc1p is not required for the spindle checkpoint. However, plc1Delta cells require a functional BUB1/BUB3-dependent spindle checkpoint for viability. PLC1 displays strong genetic interactions with genes encoding components of the inner kinetochore, including NDC10, SKP1, MIF2, CEP1, CEP3, and CTF13. Furthermore, plc1Delta cells display alterations in chromatin structure in the core centromere. Chromatin immunoprecipitation experiments indicate that Plc1p localizes to centromeric loci independently of microtubules, and accumulates at the centromeres during G(2)/M stage of cell cycle. These results are consistent with the view that Plc1p affects kinetochore function, possibly by modulating the structure of centromeric chromatin.
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