ArticleMolecular genetics and genomics : MGG2003
A candidate-gene approach to clone the sorghum Brown midrib gene encoding caffeic acid O-methyltransferase.
Article in Molecular genetics and genomics : MGG, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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59 citing papers in PubMed.
- A NovelPlants (Basel, Switzerland) · 2026Article
- Brown midrib mutants in sorghum and their applications in renewable energy production.Frontiers in plant science · 2026Review
- Hyperspectral imaging to characterize the vegetative tissue biochemical changes in response to water deficit conditions in sorghum (Frontiers in plant science · 2025Article
- Engineered reduction of S-adenosylmethionine alters lignin in sorghum.Biotechnology for biofuels and bioproducts · 2024Article
- Grass lignin: biosynthesis, biological roles, and industrial applications.Frontiers in plant science · 2024Review
- Genome-wide family prediction unveils molecular mechanisms underlying the regulation of agronomic traits inFrontiers in plant science · 2023Article
- Development of InDels markers for the identification of cytoplasmic male sterility inFrontiers in plant science · 2023Article
- Article
- Nitrogen deficiency results in changes to cell wall composition of sorghum seedlings.Scientific reports · 2021Article
- Pathogen and drought stress affect cell wall and phytohormone signaling to shape host responses in a sorghum COMT bmr12 mutant.BMC plant biology · 2021Article
- Transcriptome sequencing analysis of sorghum callus with various regeneration capacities.Planta · 2021Article
- SorghumGenes · 2021Article
- Genome-wide analysis of general phenylpropanoid and monolignol-specific metabolism genes in sugarcane.Functional & integrative genomics · 2021Article
- The Sorghum (Frontiers in plant science · 2021Article
- Large-scale genome-wide association study reveals that drought-induced lodging in grain sorghum is associated with plant height and traits linked to carbon remobilisation.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Overexpression of ferulate 5-hydroxylase increases syringyl units in Sorghum bicolor.Plant molecular biology · 2020Article
- Identification of genes from the general phenylpropanoid and monolignol-specific metabolism in two sugarcane lignin-contrasting genotypes.Molecular genetics and genomics : MGG · 2020Article
- RNAi-suppression of barley caffeic acid O-methyltransferase modifies lignin despite redundancy in the gene family.Plant biotechnology journal · 2019Article
- Biomass and Bioenergy Potential of Brown Midrib Sweet Sorghum Germplasm.Frontiers in plant science · 2019Article
- TALEN-mediated targeted mutagenesis of more than 100 COMT copies/alleles in highly polyploid sugarcane improves saccharification efficiency without compromising biomass yield.Plant biotechnology journal · 2018Article
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2 authors.
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Abstract
The brown midrib (bmr) mutants of sorghum have brown vascular tissue in the leaves and stem as a result of changes in lignin composition. The bmr mutants were generated via chemical mutagenesis with diethyl sulfate (DES) and resemble the brown midrib (bm) mutants of maize. The maize and sorghum brown midrib mutants are of particular value for the comparison of lignin biosynthesis across different, yet evolutionarily related, species. Although the sorghum brown midrib mutants were first described in 1978, none of the Brown midrib genes have been cloned. We have used a candidate-gene approach to clone the first Brown midrib gene from sorghum. Based on chemical analyses of the allelic mutants bmr12, bmr18 and bmr26, we hypothesized that these mutants had reduced activity of the lignin biosynthetic enzyme caffeic acid O-methyltransferase (COMT). After a northern analysis revealed strongly reduced expression of the COMT gene, the gene was cloned from the mutants and the corresponding wild types using PCR. In all three mutants, point mutations resulting in premature stop codons were identified: bmr12, bmr18 and bmr26 are therefore mutant alleles of the gene encoding COMT. RT-PCR indicated that all three mutants express the mutant allele, but at much lower levels relative to the wild-type controls. Molecular markers were developed for each of the three mutant alleles to facilitate the use of these mutant alleles in genetic studies and breeding programs.
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