ArticleMolecular genetics and genomics : MGG2003
Quantitative cDNA-AFLP analysis for genome-wide expression studies.
Article in Molecular genetics and genomics : MGG, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 166 citations in OpenAlex.
- The Developmental Delay of Seedlings With Cotyledons Only Confers Stress Tolerance toFrontiers in plant science · 2022Article
- Methylated site display (MSD)-AFLP, a sensitive and affordable method for analysis of CpG methylation profiles.BMC molecular biology · 2017Article
- Identification of Genes Involved in the Responses of TangorBioMed research international · 2017Article
- Suppression Subtractive Hybridization Versus Next-Generation Sequencing in Plant Genetic Engineering: Challenges and Perspectives.Molecular biotechnology · 2015Review
- Gene expression profiling by cDNA-AFLP reveals potential candidate genes for partial resistance of 'Président Roulin' against Venturia inaequalis.BMC genomics · 2014Article
- Combinatorial biosynthesis of sapogenins and saponins in Saccharomyces cerevisiae using a C-16α hydroxylase from Bupleurum falcatum.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Cryptogein-induced transcriptional reprogramming in tobacco is light dependent.Plant physiology · 2013Article
- Advances in plant gene-targeted and functional markers: a review.Plant methods · 2013Article
- Identification of differential expression genes in leaves of rice (Oryza sativa L.) in response to heat stress by cDNA-AFLP analysis.BioMed research international · 2013Article
- Comparative analysis of latex transcriptome reveals putative molecular mechanisms underlying super productivity of Hevea brasiliensis.PloS one · 2013Article
- Transcriptional profiling by cDNA-AFLP analysis showed differential transcript abundance in response to water stress in Populus hopeiensis.BMC genomics · 2012Article
- cDNA-AFLP-based genetical genomics in cotton fibers.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- Amplified fragment length polymorphism: an invaluable fingerprinting technique for genomic, transcriptomic, and epigenetic studies.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Transcriptional analysis of cell growth and morphogenesis in the unicellular green alga Micrasterias (Streptophyta), with emphasis on the role of expansin.BMC plant biology · 2011Article
- Differential gene expression and associated QTL mapping for cotton yield based on a cDNA-AFLP transcriptome map in an immortalized F2.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2011Article
- Article
- Transcriptome analysis by cDNA-AFLP of Suillus luteus Cd-tolerant and Cd-sensitive isolates.Mycorrhiza · 2011Article
- Article
- An integrated PCR colony hybridization approach to screen cDNA libraries for full-length coding sequences.PloS one · 2011Article
- Insights into the role of differential gene expression on the ecological adaptation of the snail Littorina saxatilis.BMC evolutionary biology · 2010Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An improved cDNA-AFLP method for genome-wide expression analysis has been developed. We demonstrate that this method is an efficient tool for quantitative transcript profiling and a valid alternative to microarrays. Unique transcript tags, generated from reverse-transcribed messenger RNA by restriction enzymes, were screened through a series of selective PCR amplifications. Based on in silico analysis, an enzyme combination was chosen that ensures that at least 60% of all the mRNAs were represented by an informative sequence tag. The sensitivity and specificity of the method allows one to detect poorly expressed genes and distinguish between homologous sequences. Accurate gene expression profiles were determined by quantitative analysis of band intensities, and subtle differences in transcriptional activity were revealed. A detailed screen for cell cycle-modulated genes in tobacco demonstrates the usefulness of the technology for genome-wide expression analysis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.