ArticleThe Journal of physiology2003
Aquaporin-1 and HCO3(-)-Cl- transporter-mediated transport of CO2 across the human erythrocyte membrane.
Article in The Journal of physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 70 citations in OpenAlex.
- The Evolution of Cholesterol-Rich Membrane in Oxygen Adaption: The Respiratory System as a Model.Frontiers in physiology · 2019Review
- Exploring the Potential Roles of Band 3 and Aquaporin-1 in Blood COFrontiers in physiology · 2018Review
- Are Aquaporins the Missing Transmembrane Osmosensors?The Journal of membrane biology · 2015Review
- Physiological roles of aquaporin-4 in brain.Physiological reviews · 2013Review
- Ion channels in volume regulation of clonal kidney cells.Cell proliferation · 2010Article
- Cloning and characterization of a zebrafish homologue of human AQP1: a bifunctional water and gas channel.American journal of physiology. Regulatory, integrative and comparative physiology · 2010Article
- Cancer biomarker discovery: the entropic hallmark.PloS one · 2010Article
- The spectrin-ankyrin-4.1-adducin membrane skeleton: adapting eukaryotic cells to the demands of animal life.Protoplasma · 2010Review
- Aquaporins and neurodegenerative diseases.Current neuropharmacology · 2010Article
- Exploring transmembrane diffusion pathways with molecular dynamics.Physiology (Bethesda, Md.) · 2010Review
- Nitric oxide conduction by the brain aquaporin AQP4.Proteins · 2010Article
- Expression of the gas-transporting proteins, Rh B glycoprotein and Rh C glycoprotein, in the murine lung.American journal of physiology. Lung cellular and molecular physiology · 2009Article
- Relative CO2/NH3 selectivities of AQP1, AQP4, AQP5, AmtB, and RhAG.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Extra- and intracellular unstirred layer effects in measurements of CO2 diffusion across membranes--a novel approach applied to the mass spectrometric 18O technique for red blood cells.The Journal of physiology · 2009Article
- Carbon dioxide transport through membranes.The Journal of biological chemistry · 2008Article
- Close association of water channel AQP1 with amyloid-beta deposition in Alzheimer disease brains.Acta neuropathologica · 2008Article
- Rh antigen expression during erythropoeisis: Comparison of cord and adult derived CD34 cells.Asian journal of transfusion science · 2008Article
- Does CO2 permeate through aquaporin-1?Biophysical journal · 2006Article
- What are aquaporins for?The Journal of membrane biology · 2004Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent studies have suggested that aquaporin-1 (AQP1) as well as the HCO3(-)-Cl- transporter may be involved in CO2 transport across biological membranes, but the physiological importance of this route of gas transport remained unknown. We studied CO2 transport in human red blood cell ghosts at physiological temperatures (37 degrees C). Replacement of inert with CO2-containing gas above a stirred cell suspension caused an outside-to-inside directed CO2 gradient and generated a rapid biphasic intracellular acidification. The gradient of the acidifying gas was kept small to favour high affinity entry of CO2 passing the membrane. All rates of acidification except that of the approach to physicochemical equilibrium of the uncatalysed reaction were restricted to the intracellular environment. Inhibition of carbonic anhydrase (CA) demonstrated that CO2-induced acidification required the catalytic activity of CA. Blockade of the function of either AQP1 (by HgCl2 at 65 microM) or the HCO3(-)-Cl- transporter (by DIDS at 15 microM) completely prevented fast acidification. These data indicate that, at low chemical gradients for CO2, nearly the entire CO2 transport across the red cell membrane is mediated by AQP1 and the HCO3--Cl- transporter. Therefore, these proteins may function as high affinity sites for CO2 transport across the erythrocyte membrane.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.