Evidence map›Paper›PMID 12754306›Full record

ArticleThe Journal of physiology2003

Exercise but not prostanoids enhance levels of vascular endothelial growth factor and other proliferative agents in human skeletal muscle interstitium.

Lotte Höffner, Jens Jung Nielsen, Henning Langberg, Ylva Hellsten

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed, 1 pooled it
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 1 synthesis or guideline pooled it, 106 citations in OpenAlex.

  1. Pooled it
  2. Trial
  3. Trial
  4. Trial
  5. Angiogenic Doping: Plausible Yet Difficult to Detect.Sports medicine (Auckland, N.Z.) · 2026
    Review
  6. Article
  7. Review
  8. Article
  9. Review
  10. Article
  11. Aspirin as a COX inhibitor and anti-inflammatory drug in human skeletal muscle.Journal of applied physiology (Bethesda, Md. : 1985) · 2017
    Article
  12. Advances and challenges in skeletal muscle angiogenesis.American journal of physiology. Heart and circulatory physiology · 2016
    Review
  13. Review
  14. Blood flow restriction training and the exercise pressor reflex: a call for concern.American journal of physiology. Heart and circulatory physiology · 2015
    Review
  15. Aging and the Skeletal Muscle Angiogenic Response to Exercise in Women.The journals of gerontology. Series A, Biological sciences and medical sciences · 2015
    Article
  16. Article
  17. Review
  18. Review
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Lotte HöffnerCopenhagen Muscle Research Centre, Institute for Exercise and Sport Science, Copenhagen University, and Sports Medicine Research Unit, Bispebjerg Hospital, Denmark.
Jens Jung Nielsen
Henning Langberg
Ylva Hellsten
Bispebjerg Hospital · DK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the present study we examined whether exercise and prostanoids have an effect on the muscle interstitial concentration of vascular endothelial growth factor (VEGF) and on the proliferative effect of muscle interstitial fluid. Dialysate from resting and exercising human skeletal muscle, obtained either during control conditions or during cyclooxygenase inhibition, was examined for its content of VEGF and for its effect on endothelial cell proliferation. Microdialysis probes with high (960 kDa) and low (5 kDa) molecular-mass cut-off membranes were placed in the vastus lateralis muscle of healthy young males. The subjects performed one-legged knee extensions (20 W). The concentration of VEGF in the 960 kDa dialysate was greater (P < 0.05) during exercise compared to at rest (67 +/- 28 vs. 230 +/- 22 pg ml-1). The rate of endothelial cell proliferation was 2.7-fold higher (P < 0.05) with the 960 kDa dialysate from resting muscle than with perfusate and was 5.8-fold higher (P < 0.05) than the perfusate value with dialysate from exercising muscle. VEGF was not enhanced with exercise in the 5 kDa dialysate, yet the exercise dialysate induced a 1.9-fold higher (P < 0.05) proliferation than the resting dialysate. Cyclooxygenase inhibition did not affect the VEGF concentration or the proliferating effect of the dialysates (P > 0.05). This study demonstrates for the first time that VEGF is present in the interstitium of human skeletal muscle and that exercise enhances the interstitial concentration of VEGF and of other, as yet unidentified, angiogenic compounds. Products of cyclooxygenase do not appear to have an effect on the release of VEGF or other proliferative agents in human skeletal muscle.

Indexed as

6-Ketoprostaglandin F1 alphaAdultCell DivisionCyclooxygenase InhibitorsDinoprostoneEndothelium, VascularExerciseHumansLegMaleMicrodialysisMuscle, SkeletalProstaglandinsTissue ExtractsVascular Endothelial Growth Factor A6-Ketoprostaglandin F1 alphaCyclooxygenase InhibitorsDinoprostoneProstaglandinsTissue ExtractsVascular Endothelial Growth Factor A

Identifiers

PMID12754306
PMCPMC2343029
OpenAlexW2138441948

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.