ArticleJournal of bacteriology2003
Contribution of aggregation-promoting factor to maintenance of cell shape in Lactobacillus gasseri 4B2.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 81 citations in OpenAlex.
- Modulation of Mouse Dendritic Cells In Vitro by Lactobacillus gasseri Postbiotic Proteins.Probiotics and antimicrobial proteins · 2025Article
- Lactobacillus crispatus S-layer proteins modulate innate immune response and inflammation in the lower female reproductive tract.Nature communications · 2024Article
- Insights From theFrontiers in microbiology · 2022Review
- Probiotic properties of lactic acid bacteria isolated from traditionally prepared dry starters of the Eastern Himalayas.World journal of microbiology & biotechnology · 2021Article
- Molecular insights into probiotic mechanisms of action employed against intestinal pathogenic bacteria.Gut microbes · 2020Review
- Techno-functional characterization of indigenousCurrent research in food science · 2020Article
- Deletion of S-Layer Associated Ig-Like Domain Protein Disrupts theFrontiers in microbiology · 2020Article
- A Cell Surface Aggregation-Promoting Factor from Lactobacillus gasseri Contributes to Inhibition of Trichomonas vaginalis Adhesion to Human Vaginal Ectocervical Cells.Infection and immunity · 2018Article
- Genomic and physiological analyses of an indigenous strain, Enterococcus faecium 17OM39.Functional & integrative genomics · 2018Article
- The aggregation-promoting factor in Lactobacillus delbrueckii ssp. bulgaricus: confirmation of the presence and expression of the apf gene and in silico analysis of the corresponding protein.World journal of microbiology & biotechnology · 2018Article
- Extractable Bacterial Surface Proteins in Probiotic-Host Interaction.Frontiers in microbiology · 2018Review
- Article
- Aggregation factor as an inhibitor of bacterial binding to gut mucosa.Microbial ecology · 2014Article
- Generation of food-grade recombinant Lactobacillus casei delivering Myxococcus xanthus prolyl endopeptidase.Applied microbiology and biotechnology · 2014Article
- Lactobacillus gasseri SBT2055 reduces infection by and colonization of Campylobacter jejuni.PloS one · 2014Article
- An extracellular Serine/Threonine-rich protein from Lactobacillus plantarum NCIMB 8826 is a novel aggregation-promoting factor with affinity to mucin.Applied and environmental microbiology · 2013Article
- Lactobacillus surface layer proteins: structure, function and applications.Applied microbiology and biotechnology · 2013Review
- Biological characteristics and probiotic effect of Leuconostoc lactis strain isolated from the intestine of black porgy fish.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2013Article
- Inhibition of Staphylococcus aureus by lysostaphin-expressing Lactobacillus plantarum WCFS1 in a modified genital tract secretion medium.Applied and environmental microbiology · 2011Article
- Characterization of Putative Adhesion Genes in the Potentially Probiotic Strain Lactobacillus plantarum BFE 5092.Probiotics and antimicrobial proteins · 2011Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aggregation-promoting factor (APF) was originally described as a protein involved in the conjugation and autoaggregation of Lactobacillus gasseri 4B2, whose corresponding apf gene was cloned and sequenced. In this report, we identified and sequenced an additional apf gene located in the region upstream of the previously published one. Inactivation of both apf genes was unsuccessful, indicating that APF function may be essential for the cell. Overproduction of APF proteins caused drastic alteration in the cell shape of this strain. These cells were irregular, twisted, enlarged, and tightly bound in unbreakable clumps of chains. Down-regulation of APF synthesis was achieved by cloning of the apf2 promoter region on a high-copy-number plasmid, which recruited a putative apf activator. As a consequence, the shape of the corresponding recombinant cells was elongated (filamentous) and cell division sites were no longer visible. None of the induced changes in APF production levels was clearly correlated with modifications of the aggregation phenotype. This report shows, for the first time, that APF proteins are mainly critical for L. gasseri 4B2 cell shape maintenance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.