ArticleJournal of bacteriology2003
Functional subsets of the virB type IV transport complex proteins involved in the capacity of Agrobacterium tumefaciens to serve as a recipient in virB-mediated conjugal transfer of plasmid RSF1010.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Article
- Gain and loss of phototrophic genes revealed by comparison of two Citromicrobium bacterial genomes.PloS one · 2012Article
- An Agrobacterium VirB10 mutation conferring a type IV secretion system gating defect.Journal of bacteriology · 2011Article
- Phylogenomics reveals a diverse Rickettsiales type IV secretion system.Infection and immunity · 2010Review
- Biological diversity of prokaryotic type IV secretion systems.Microbiology and molecular biology reviews : MMBR · 2009Review
- Small heat-shock protein HspL is induced by VirB protein(s) and promotes VirB/D4-mediated DNA transfer in Agrobacterium tumefaciens.Microbiology (Reading, England) · 2009Article
- Interactions between Brucella suis VirB8 and its homolog TraJ from the plasmid pSB102 underline the dynamic nature of type IV secretion systems.Journal of bacteriology · 2009Article
- Structure of a type IV secretion system core complex.Science (New York, N.Y.) · 2009Article
- Article
- Environmental mimics and the Lvh type IVA secretion system contribute to virulence-related phenotypes of Legionella pneumophila.Infection and immunity · 2007Article
- Functional and topological characterization of novel components of the comB DNA transformation competence system in Helicobacter pylori.Journal of bacteriology · 2006Article
- The Brucella suis type IV secretion system assembles in the cell envelope of the heterologous host Agrobacterium tumefaciens and increases IncQ plasmid pLS1 recipient competence.Infection and immunity · 2006Article
- Spatial location and requirements for the assembly of the Agrobacterium tumefaciens type IV secretion apparatus.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Biogenesis, architecture, and function of bacterial type IV secretion systems.Annual review of microbiology · 2005Review
- Energetic components VirD4, VirB11 and VirB4 mediate early DNA transfer reactions required for bacterial type IV secretion.Molecular microbiology · 2004Article
- Type IV secretion: the Agrobacterium VirB/D4 and related conjugation systems.Biochimica et biophysica acta · 2004Review
- Osa protein constitutes a strong oncogenic suppression system that can block vir-dependent transfer of IncQ plasmids between Agrobacterium cells and the establishment of IncQ plasmids in plant cells.Journal of bacteriology · 2004Article
- VirB1 orthologs from Brucella suis and pKM101 complement defects of the lytic transglycosylase required for efficient type IV secretion from Agrobacterium tumefaciens.Journal of bacteriology · 2004Article
- A subassembly of R27-encoded transfer proteins is dependent on TrhC nucleoside triphosphate-binding motifs for function but not formation.Journal of bacteriology · 2004Article
- Detection of evolutionarily stable fragments of cellular pathways by hierarchical clustering of phyletic patterns.Genome biology · 2004Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The virB-encoded type IV transport complex of Agrobacterium tumefaciens mediates the transfer of DNA and proteins into plant cells, as well as the conjugal transfer of IncQ plasmids, such as RSF1010, between Agrobacterium strains. While several studies have indicated that there are physical interactions among the 11 VirB proteins, the functional significance of the interactions has been difficult to establish since all of the proteins are required for substrate transfer. Our previous studies, however, indicated that although all of the VirB proteins are required for the capacity of a strain to serve as an RSF1010 donor, only a subset of these proteins in the recipient is necessary to increase the conjugal frequency by 3 to 4 logs. The roles of particular groups of VirB proteins in this increased recipient activity were examined in the study reported here. Examination of the expression of subgroups of virB genes revealed that translation of virB6 is necessary for expression of downstream open reading frames. Expression of limited subsets of the VirB proteins in a recipient strain lacking the Ti plasmid revealed that the VirB7 to VirB10 proteins yield a subcomplex that is functional in the recipient assay but that the VirB1 to VirB4 proteins, as a group, dramatically increase this activity in strains expressing VirB7 to VirB10. Finally, the membrane distribution and cross-linking patterns of VirB10, but not of VirB8 or VirB9, in a strain expressing only VirB7 to VirB10 are significantly altered compared to the patterns of the wild type. These characteristics are, however, restored to the wild-type status by coexpression of VirB1 to VirB3. Taken together, these results define subsets of type IV transport complex proteins that are critical in allowing a strain to participate as a recipient in virB-mediated conjugal RSF1010 transfer.
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