ArticlePharmaceutical research2003
Expression and functional involvement of organic anion transporting polypeptide subtype 3 (Slc21a7) in rat choroid plexus.
Article in Pharmaceutical research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 38 citations in OpenAlex.
- Thyroxine (TFrontiers in neurology · 2017Article
- Drug transporters in the central nervous system.Clinical pharmacokinetics · 2015Review
- Efflux transporters in blood-brain interfaces of the developing brain.Frontiers in neuroscience · 2015Review
- Intestinal absorption of forsythoside A in in situ single-pass intestinal perfusion and in vitro Caco-2 cell models.Acta pharmacologica Sinica · 2012Article
- Expression of Tight Junction and Drug Efflux Transporter Proteins in an in vitro Model of Human Blood-Brain Barrier.Frontiers in psychiatry · 2012Article
- Expression Profiling of Solute Carrier Gene Families at the Blood-CSF Barrier.Frontiers in pharmacology · 2012Article
- Molecular biology of the blood-brain and the blood-cerebrospinal fluid barriers: similarities and differences.Fluids and barriers of the CNS · 2011Article
- Thyroid hormone transporters in the brain.Cerebellum (London, England) · 2008Review
- The distribution of the anti-HIV drug, tenofovir (PMPA), into the brain, CSF and choroid plexuses.Cerebrospinal fluid research · 2006Article
- Active efflux across the blood-brain barrier: role of the solute carrier family.NeuroRx : the journal of the American Society for Experimental NeuroTherapeutics · 2005Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeIt has been shown that transport(s) are involved in the uptake of estradiol 17beta glucuronide (E217betaG) by the choroid plexus (CP). The purpose of this study is to compare the substrate specificity of the transporter in the CP with those of rat organic anion transporting polypeptide 1 (rOatp1) and rOatp3.
methodsThe expression of rOatp1 and rOatp3 in rat CP was confirmed by RT-PCR and Western blot analyses. The substrate specificity of rOatp1 and rOatp3 was compared using cDNA-transfected LLC-PK1 cells. The uptake of E217betaG by rat isolated CP was determined by centrifugal filtration technique.
resultsPCR analyses demonstrated that the mRNA expression of rOatp3 was abundant in the CP, whereas that of rOatp1 was low. Immunohistochemical staining revealed that rOatp3 is expressed on the apical membrane of the CP. Kinetic parameters (Km and Ki values) of rOatp3 were similar to those for rOatp1. The results of mutual inhibition study suggest that E217betaG and taurocholate share the same mechanism in the CP. Corticosterone, estrone-3-sulfate and indomethacin are moderate inhibitors, but no effects by digoxin, p-aminohippurate, benzylpenicillin and cimetidine were observed.
conclusionsrOatp3 is most possible candidate transporter involved in the uptake of organic anions on the brush border membrane of the choroid epithelial cells.
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