ArticlePlanta2003
Molecular characterization and post-transcriptional regulation of ME1, a type-I ribosome-inactivating protein from Mirabilis expansa.
Article in Planta, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed, 25 citations in OpenAlex.
- The role of enzymatic activities of antiviral proteins from plants for action against plant pathogens.3 Biotech · 2020Review
- The Pokeweed Leaf mRNA Transcriptome and Its Regulation by Jasmonic Acid.Frontiers in plant science · 2016Article
- Ribosome-inactivating and related proteins.Toxins · 2015Review
- Protein synthesis inhibition activity by strawberry tissue protein extracts during plant life cycle and under biotic and abiotic stresses.International journal of molecular sciences · 2013Article
- Genome-wide survey of the RIP domain family in Oryza sativa and their expression profiles under various abiotic and biotic stresses.Plant molecular biology · 2008Article
- Cloning and expression of antiviral/ribosome-inactivating protein from Bougainvillea xbuttiana.Journal of biosciences · 2008Article
- Ribosome-inactivating proteins in plant biology.Planta · 2004Review
- Isolation and characterization of an RIP (ribosome-inactivating protein)-like protein from tobacco with dual enzymatic activity.Plant physiology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ribosome-inactivating proteins (RIPs) are N-glycosidases that remove a specific adenine from the sarcin/ricin (S/R) loop of the large rRNA, thus arresting protein synthesis at the translocation step. In the present study, ME1, a type-1 RIP, was cloned and sequenced from storage roots of Mirabilis expansa (Ruiz & Pavon). The full-length cDNA sequence of ME1 has 1,129 nucleotides with an open reading frame of 951 nucleotides representing 317 amino acids. Nucleotide analysis revealed that the N-terminal region of ME1 was cleaved, and the mature protein started at amino acid 34. ME1 showed very close similarities to MAP and MAP-4 from Mirabilis jalapa. Southern blot analysis revealed the presence of two homologous genes for ME1 cDNA in M. expansa. Northern blot analysis showed high levels of ME1 transcripts in primary and storage roots. Interestingly, jasmonic acid induced ME1 transcript expression in cell suspension cultures of M. expansa; however, the production of ME1 protein was not enhanced as observed by Western blot analysis. Our data suggest that ME1 has the ability to depurinate its own mRNA, thus inhibiting its translation. These observations suggest a possible mechanism by which ME1 protein levels are post-transcriptionally regulated.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.