ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2003
AFLP analysis of genetic variability in New Guinea impatiens.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed, 28 citations in OpenAlex.
- Genetic diversity and population differentiation of traditional fonio millet (Digitaria spp.) landraces from different agro-ecological zones of West Africa.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2007Article
- Amplified fragment length polymorphism for genetic diversity assessment in globe artichoke.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2004Article
- Genetic relationships of Aglaonema species and cultivars inferred from AFLP markers.Annals of botany · 2004Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
New Guinea impatiens ( Impatiens hawkeri) is an economically important floral crop, however, little work has been conducted to further our understanding of the genetics of this crop. In this study, we used amplified fragment length polymorphism (AFLP) technology to investigate the level of polymorphism present among 41 commercial cultivars of New Guinea impatiens, study their genetic relatedness, and assess the genetic diversity in this material. An efficient DNA extraction protocol was developed, and a total of 48 EcoRI and MseI primer combinations were used for PCR amplification. Amplification products were then subjected to polyacrylamide gel electrophoresis. The AFLP analysis showed that all 41 cultivars generated between 73 and 130 scoreable polymorphic bands per primer combination. Gower's Genetic Dissimilarity estimates for the entire set of cultivars ranged between 0.940 and 0.488. A dendogram was generated from these dissimilarity data that revealed four groupings among these 41 cultivars. The implications of these results on genotypic variation, genetic relationships, and genetic diversity in New Guinea impatiens will be discussed.
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