ArticleCurrent genetics2003
Polyadenylation of rRNA- and tRNA-based yeast transcripts cleaved by internal ribozyme activity.
Article in Current genetics, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
4 citing papers in PubMed.
- UV Laser-Induced, Time-Resolved Transcriptome Responses ofG3 (Bethesda, Md.) · 2019Article
- Construction and characterization of an improved DNA-launched infectious clone of duck hepatitis a virus type 1.Virology journal · 2017Article
- Article
- A synthetic A tail rescues yeast nuclear accumulation of a ribozyme-terminated transcript.RNA (New York, N.Y.) · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polyadenylation, an important step in 3' end-processing of mRNA in eukaryotes, results in a poly(A) tail that ensures RNA transport into the cytoplasm and subsequent translation. Addition of a poly(A) tail is restricted to transcripts that are synthesized by RNA polymerase II. Here, we demonstrate that the 3' ends of yeast transcripts based on rRNA and tRNA, respectively, can be polyadenylated in vivo. The transcripts were modified by insertion of a self-cleaving hammerhead ribozyme sequence in the corresponding gene. Both the rDNA-based transcript and the tRNA transcript were cleaved efficiently by the hammerhead ribozyme, resulting in two stable cleavage products. The 5' cleavage product was found to be polyadenylated in both cases. This demonstrates that, in yeast, transcripts that are usually synthesized by RNA polymerase I or III can be polyadenylated if the 3' end of the transcript has been generated independently by a ribozyme.
Indexed as
Identifiers
12748813What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.