ArticleMolecular and cellular biology2003
In vitro import of a nuclearly encoded tRNA into mitochondria of Solanum tuberosum.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 61 citations in OpenAlex.
- Rewiring of Aminoacyl-tRNA Synthetase Localization and Interactions in Plants With Extensive Mitochondrial tRNA Gene Loss.Molecular biology and evolution · 2023Article
- Rapid Shifts in Mitochondrial tRNA Import in a Plant Lineage with Extensive Mitochondrial tRNA Gene Loss.Molecular biology and evolution · 2021Article
- tRNA-Dependent Import of a Transit Sequence-Less Aminoacyl-tRNA Synthetase (LeuRS2) into the Mitochondria of Arabidopsis.International journal of molecular sciences · 2021Article
- Non-coding RNA Regulated Cross-Talk Between Mitochondria and Other Cellular Compartments.Frontiers in cell and developmental biology · 2021Review
- High mitochondrial mutation rates inBiology letters · 2020Article
- Import of Non-Coding RNAs into Human Mitochondria: A Critical Review and Emerging Approaches.Cells · 2019Review
- tRNA biology in mitochondria.International journal of molecular sciences · 2015Review
- Importance of adenosine-to-inosine editing adjacent to the anticodon in an Arabidopsis alanine tRNA under environmental stress.Nucleic acids research · 2013Article
- The import of the transcription factor STAT3 into mitochondria depends on GRIM-19, a component of the electron transport chain.The Journal of biological chemistry · 2013Article
- Organelle trafficking of chimeric ribozymes and genetic manipulation of mitochondria.Nucleic acids research · 2011Article
- A protein shuttle system to target RNA into mitochondria.Nucleic acids research · 2011Article
- In vivo study in Trypanosoma brucei links mitochondrial transfer RNA import to mitochondrial protein import.EMBO reports · 2011Article
- Import of tRNAs and aminoacyl-tRNA synthetases into mitochondria.Current genetics · 2009Review
- Mammalian mitochondria have the innate ability to import tRNAs by a mechanism distinct from protein import.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Proton-guided movements of tRNA within the Leishmania mitochondrial RNA import complex.Nucleic acids research · 2008Article
- Delivery of drugs and macromolecules to mitochondria.Advanced drug delivery reviews · 2007Review
- The voltage-dependent anion channel, a major component of the tRNA import machinery in plant mitochondria.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- A glycolytic enzyme, enolase, is recruited as a cofactor of tRNA targeting toward mitochondria in Saccharomyces cerevisiae.Genes & development · 2006Article
- Saccharomyces cerevisiae imports the cytosolic pathway for Gln-tRNA synthesis into the mitochondrion.Genes & development · 2005Article
- Allosteric regulation of tRNA import: interactions between tRNA domains at the inner membrane of Leishmania mitochondria.Nucleic acids research · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Some of the mitochondrial tRNAs of higher plants are nuclearly encoded and imported into mitochondria. The import of tRNAs encoded in the nucleus has been shown to be essential for proper protein translation within mitochondria of a variety of organisms. Here, we report the development of an in vitro assay for import of nuclearly encoded tRNAs into plant mitochondria. This in vitro system utilizes isolated mitochondria from Solanum tuberosum and synthetic tRNAs transcribed from cloned nuclear tRNA genes. Although incubation of radioactively labeled in vitro-transcribed tRNA(Ala), tRNA(Phe), and tRNA(Met-e) with isolated potato mitochondria resulted in importation, as measured by nuclease protection, the amount of tRNA transcripts protected at saturation was at least five times higher for tRNA(Ala) than for the two other tRNAs. This difference in in vitro saturation levels of import is consistent with the in vivo localization of these tRNAs, since cytosolic tRNA(Ala) is naturally imported into potato mitochondria whereas tRNA(Phe) and tRNA(Met-e) are not. Characterization of in vitro tRNA import requirements indicates that mitochondrial tRNA import proceeds in the absence of any added cytosolic protein fraction, involves at least one protein component on the surface of mitochondria, and requires ATP-dependent step(s) and a membrane potential.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.