ArticleMolecular biotechnology2003
A novel method for preparing single-stranded DNA for pyrosequencing.
Article in Molecular biotechnology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed.
- On-Flow Synthesis of Co-Polymerizable Oligo-Microspheres and Application in ssDNA Amplification.PloS one · 2016Article
- Methods for the preparation of large quantities of complex single-stranded oligonucleotide libraries.PloS one · 2014Article
- Direct amplification of single-stranded DNA for pyrosequencing using linear-after-the-exponential (LATE)-PCR.Analytical biochemistry · 2006Article
- Pyrosequencing: sequence typing at the speed of light.Molecular biotechnology · 2004Review
- Genotypic characterization of Neisseria meningitidis using Pyrosequencing.Molecular biotechnology · 2004Review
- Rapid detection and estimation by pyrosequencing of 23S rRNA genes with a single nucleotide polymorphism conferring linezolid resistance in Enterococci.Antimicrobial agents and chemotherapy · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pyrosequencing technology is a powerful genotyping tool that requires the generation of single stranded DNA. Currently, two simple, solid-phase-based methods are available for this, but they require special equipment, they are not automated, and they are relatively expensive because of the need for biotinylated polymerase chain reaction primers. In this article, an enzymatic liquid-phase method for the generation of high-quality, single-stranded DNA, and its novel use for Pyrosequencing are described. The method has also been fully automated.
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12746558What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.