ArticlePlant physiology2003
Identification of novel mitochondrial protein components of Chlamydomonas reinhardtii. A proteomic approach.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 115 citations in OpenAlex.
- Structural dynamics of mitochondrial ATP synthase inbioRxiv : the preprint server for biology · 2025Article
- Article
- Supramolecular associations between atypical oxidative phosphorylation complexes of Euglena gracilis.Journal of bioenergetics and biomembranes · 2021Article
- Strategies to Study Dark Growth Deficient or Slower Mutants in Chlamydomonas reinhardtii.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Thermotaxis in Chlamydomonas is brought about by membrane excitation and controlled by redox conditions.Scientific reports · 2018Article
- Review
- Structure and mechanism of the ATP synthase membrane motor inferred from quantitative integrative modeling.The Journal of general physiology · 2016Article
- Lack of FTSH4 Protease Affects Protein Carbonylation, Mitochondrial Morphology, and Phospholipid Content in Mitochondria of Arabidopsis: New Insights into a Complex Interplay.Plant physiology · 2016Article
- A refined genome-scale reconstruction of Chlamydomonas metabolism provides a platform for systems-level analyses.The Plant journal : for cell and molecular biology · 2015Article
- Establishing Chlamydomonas reinhardtii as an industrial biotechnology host.The Plant journal : for cell and molecular biology · 2015Review
- Alternative oxidase expression in the mouse enables bypassing cytochrome c oxidase blockade and limits mitochondrial ROS overproduction.PLoS genetics · 2013Article
- In silico survey of the mitochondrial protein uptake and maturation systems in the brown alga Ectocarpus siliculosus.PloS one · 2011Article
- Knock-down of the COX3 and COX17 gene expression of cytochrome c oxidase in the unicellular green alga Chlamydomonas reinhardtii.Plant molecular biology · 2010Article
- Biochemical and structural studies of the large Ycf4-photosystem I assembly complex of the green alga Chlamydomonas reinhardtii.The Plant cell · 2009Article
- The fully-active and structurally-stable form of the mitochondrial ATP synthase of Polytomella sp. is dimeric.Journal of bioenergetics and biomembranes · 2009Article
- Eukaryotic complex I: functional diversity and experimental systems to unravel the assembly process.Molecular genetics and genomics : MGG · 2008Review
- Nuclear and mitochondrial subunits from the white shrimp Litopenaeus vannamei F(0)F(1) ATP-synthase complex: cDNA sequence, molecular modeling, and mRNA quantification of atp9 and atp6.Journal of bioenergetics and biomembranes · 2008Article
- Treasure hunting in the Chlamydomonas genome.Genetics · 2008Article
- Dimer ribbons of ATP synthase shape the inner mitochondrial membrane.The EMBO journal · 2008Article
- Genome analysis and its significance in four unicellular algae, Cyanidioschyzon [corrected] merolae, Ostreococcus tauri, Chlamydomonas reinhardtii, and Thalassiosira pseudonana.Journal of plant research · 2008Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pure mitochondria of the photosynthetic alga Chlamydomonas reinhardtii were analyzed using blue native-polyacrylamide gel electrophoresis (BN-PAGE). The major oxidative phosphorylation complexes were resolved: F(1)F(0)-ATP synthase, NADH-ubiquinone oxidoreductase, ubiquinol-cytochrome c reductase, and cytochrome c oxidase. The oligomeric states of these complexes were determined. The F(1)F(0)-ATP synthase runs exclusively as a dimer, in contrast to the C. reinhardtii chloroplast enzyme, which is present as a monomer and subcomplexes. The sequence of a 60-kD protein, associated with the mitochondrial ATP synthase and with no known counterpart in any other organism, is reported. This protein may be related to the strong dimeric character of the algal F(1)F(0)-ATP synthase. The oxidative phosphorylation complexes resolved by BN-PAGE were separated into their subunits by second dimension sodium dodecyl sulfate-PAGE. A number of polypeptides were identified mainly on the basis of their N-terminal sequence. Core I and II subunits of complex III were characterized, and their proteolytic activities were predicted. Also, the heterodimeric nature of COXIIA and COXIIB subunits in cytochrome c oxidase was demonstrated. Other mitochondrial proteins like the chaperone HSP60, the alternative oxidase, the aconitase, and the ADP/ATP carrier were identified. BN-PAGE was also used to approach the analysis of the major chloroplast protein complexes of C. reinhardtii.
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