ArticlePlant physiology2003
Chimeric RNA/DNA oligonucleotide-based site-specific modification of the tobacco acetolactate syntase gene.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 70 citations in OpenAlex.
- Application of Antisense Oligonucleotides as an Alternative Approach for Gene Expression Control and Functional Studies.International journal of molecular sciences · 2025Review
- Oligo-Not Only for Silencing: Overlooked Potential for Multidirectional Action in Plants.International journal of molecular sciences · 2023Review
- Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat.Frontiers in genetics · 2023Review
- Application of Genome Editing in Tomato Breeding: Mechanisms, Advances, and Prospects.International journal of molecular sciences · 2021Review
- CRISPR/Cas9-mediated homologous recombination in tobacco.Plant cell reports · 2019Article
- Transgene-Free Genome Editing in Tomato and Potato Plants UsingInternational journal of molecular sciences · 2019Article
- Editing the Genome Without Double-Stranded DNA Breaks.ACS chemical biology · 2018Review
- Relaxed chromatin induced by histone deacetylase inhibitors improves the oligonucleotide-directed gene editing in plant cells.Journal of plant research · 2018Article
- Progress of targeted genome modification approaches in higher plants.Plant cell reports · 2016Review
- Oligonucleotide-directed mutagenesis for precision gene editing.Plant biotechnology journal · 2016Review
- The Regulatory Status of Genome-edited Crops.Plant biotechnology journal · 2016Review
- CRISPR-Cas9: Tool for Qualitative and Quantitative Plant Genome Editing.Frontiers in plant science · 2016Review
- Article
- An attempt to detect siRNA-mediated genomic DNA modification by artificially induced mismatch siRNA in Arabidopsis.PloS one · 2013Article
- Use of chimeric DNA-RNA primers in quantitative PCR for detection of Ehrlichia canis and Babesia canis.Applied and environmental microbiology · 2009Article
- Oligonucleotide-directed gene repair in wheat using a transient plasmid gene repair assay system.Plant cell reports · 2006Article
- Modification of endogenous natural genes by gene targeting in rice and other higher plants.Plant molecular biology · 2005Article
- Targeted site-directed mutagenesis of a heme oxygenase locus by gene replacement in the moss Ceratodon purpureus.Planta · 2005Article
- Spontaneous mutation frequency in plants obscures the effect of chimeraplasty.Plant molecular biology · 2003Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single amino acid substitutions at either of two crucial positions in acetolactate synthase (ALS) result in a chlorsulfuron-insensitive form of this enzyme and, as a consequence, a herbicide-resistant phenotype. Here, we describe the successful in vivo targeting of endogenous tobacco (Nicotiana tabacum) ALS genes using chimeric RNA/DNA and all-DNA oligonucleotides at two different locations. Similar number of conversion events with two different chimeras indicates the absence of restricting influence of genomic target sequence on the gene repair in tobacco. Chlorsulfuron-resistant plants were regenerated from calli after mesophyll protoplast electroporation or leaf tissue particle bombardment with these specifically constructed chimeras. Sequence analysis and enzyme assays proved the resulting alterations to ALS at both DNA and protein levels. Furthermore, foliar application of chlorsulfuron confirmed the development of resistant phenotypes. Lines with proline-196-alanine, threonine, glutamine, or serine substitutions or with tryptophan-573-leucine substitutions were highly resistant at both cellular and whole plant levels, whereas lines with proline-196-leucine substitutions were less resistant. The stability of these modifications was demonstrated by the continuous growth of calli on chlorsulfuron-containing medium and by the transmission of herbicide resistance to progeny in a Mendelian manner. Ability of haploid state to promote chimera-mediated conversions is discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.