ArticlePlant physiology2003
A novel family in Medicago truncatula consisting of more than 300 nodule-specific genes coding for small, secreted polypeptides with conserved cysteine motifs.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 170 papers.
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Who cites it
170 citing papers in PubMed, 375 citations in OpenAlex.
- ESN3, a nodule-specific small peptide essential for symbiotic nitrogen fixation in Medicago truncatula.The New phytologist · 2026Article
- Symbiotic peptides modulate rhizobial physiology without terminal differentiation.Science advances · 2026Article
- A Chickpea NCR Peptide Suppresses Postharvest Alternaria alternata Infection by Disrupting Fungal Membranes, Mitochondria and Respiration.Molecular plant pathology · 2026Article
- Engineering Symbiotic Nitrogen Fixation for Agriculture: Predominant Role of Host Plants and Fine-Tuning Regulation.Plants (Basel, Switzerland) · 2026Article
- BacA(SbmA) importer of legume symbiotic NCR peptides: Protein architecture, function, and evolutionary implications.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- A plant-derived antimicrobial peptide with multiple mechanisms of action exhibiting antibacterial and antibiofilm activities comparable to or superior to polymyxin B.Current research in microbial sciences · 2026Article
- Expression profile of cystatin gene family in alfalfa (Medicago sativa L.) related to biotic and abiotic stress response.BMC genomics · 2025Article
- Protein import into bacterial endosymbionts and evolving organelles.The FEBS journal · 2025Review
- Multifaceted antimicrobial mechanisms of NCR147-derived peptides fromFrontiers in microbiology · 2025Article
- Antagonism within mutualism: host control of symbionts through nodule-specific antimicrobial peptides.Frontiers in microbiology · 2025Review
- A mitochondrial metalloprotease FtsH4 is required for symbiotic nitrogen fixation in Lotus japonicus nodules.Scientific reports · 2024Article
- Two members of a Nodule-specific Cysteine-Rich (NCR) peptide gene cluster are required for differentiation of rhizobia in Medicago truncatula nodules.The Plant journal : for cell and molecular biology · 2024Article
- Genome-Wide Identification of the SPP/SPPL Gene Family andInternational journal of molecular sciences · 2024Article
- Exploring the role of symbiotic modifier peptidases in the legume - rhizobium symbiosis.Archives of microbiology · 2024Review
- Autophagy and Symbiosis: Membranes, ER, and Speculations.International journal of molecular sciences · 2024Review
- Signaling in Legume-Rhizobia Symbiosis.International journal of molecular sciences · 2023Review
- Targeted mutagenesis of Medicago truncatula Nodule-specific Cysteine-Rich (NCR) genes using the Agrobacterium rhizogenes-mediated CRISPR/Cas9 system.Scientific reports · 2023Article
- A protease and a lipoprotein jointly modulate the conserved ExoR-ExoS-ChvI signaling pathway critical in Sinorhizobium meliloti for symbiosis with legume hosts.PLoS genetics · 2023Article
- Evolution of antimicrobial cysteine-rich peptides in plants.Plant cell reports · 2023Article
- A signal peptide peptidase is required for ER-symbiosome proximal association and protein secretion.Nature communications · 2023Article
110 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcriptome analysis of Medicago truncatula nodules has led to the discovery of a gene family named NCR (nodule-specific cysteine rich) with more than 300 members. The encoded polypeptides were short (60-90 amino acids), carried a conserved signal peptide, and, except for a conserved cysteine motif, displayed otherwise extensive sequence divergence. Family members were found in pea (Pisum sativum), broad bean (Vicia faba), white clover (Trifolium repens), and Galega orientalis but not in other plants, including other legumes, suggesting that the family might be specific for galegoid legumes forming indeterminate nodules. Gene expression of all family members was restricted to nodules except for two, also expressed in mycorrhizal roots. NCR genes exhibited distinct temporal and spatial expression patterns in nodules and, thus, were coupled to different stages of development. The signal peptide targeted the polypeptides in the secretory pathway, as shown by green fluorescent protein fusions expressed in onion (Allium cepa) epidermal cells. Coregulation of certain NCR genes with genes coding for a potentially secreted calmodulin-like protein and for a signal peptide peptidase suggests a concerted action in nodule development. Potential functions of the NCR polypeptides in cell-to-cell signaling and creation of a defense system are discussed.
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