ArticleOncogene2003
Identification of cJun-responsive genes in Rat-1a cells using multiple techniques: increased expression of stathmin is necessary for cJun-mediated anchorage-independent growth.
Article in Oncogene, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- Article
- PTEN loss promotes oncogenic function of STMN1 via PI3K/AKT pathway in lung cancer.Scientific reports · 2021Article
- c-Jun enhances intestinal epithelial restitution after wounding by increasing phospholipase C-γ1 transcription.American journal of physiology. Cell physiology · 2017Article
- Article
- Genetic analysis of genes causing hypertension and stroke in spontaneously hypertensive rats: Gene expression profiles in the kidneys.International journal of molecular medicine · 2015Article
- p21-activated kinase 3 (PAK3) is an AP-1 regulated gene contributing to actin organisation and migration of transformed fibroblasts.PloS one · 2013Article
- Stathmin regulates keratinocyte proliferation and migration during cutaneous regeneration.PloS one · 2013Article
- Mammary gland selective excision of c-jun identifies its role in mRNA splicing.Cancer research · 2012Article
- Transcriptional tools: Small molecules for modulating CBP KIX-dependent transcriptional activators.Biopolymers · 2011Article
- Emerging roles of ATF2 and the dynamic AP1 network in cancer.Nature reviews. Cancer · 2010Review
- Inhibition of AP-1 transcriptional activity blocks the migration, invasion, and experimental metastasis of murine osteosarcoma.The American journal of pathology · 2009Article
- Simultaneous blockade of AP-1 and phosphatidylinositol 3-kinase pathway in non-small cell lung cancer cells.British journal of cancer · 2008Article
- Clinicopathological significance of expression of p-c-Jun, TCF4 and beta-Catenin in colorectal tumors.BMC cancer · 2008Article
- A novel role for jun N-terminal kinase signaling in olfactory sensory neuronal death.Molecular and cellular neurosciences · 2008Article
- Disruption of c-Jun reduces cellular migration and invasion through inhibition of c-Src and hyperactivation of ROCK II kinase.Molecular biology of the cell · 2008Article
- Growth inhibition of non-small cell lung cancer cells by AP-1 blockade using a cJun dominant-negative mutant.British journal of cancer · 2008Article
- Cell fate determination factor DACH1 inhibits c-Jun-induced contact-independent growth.Molecular biology of the cell · 2007Article
- Inducible overexpression of c-Jun in MCF7 cells causes resistance to vinblastine via inhibition of drug-induced apoptosis and senescence at a step subsequent to mitotic arrest.Biochemical pharmacology · 2007Article
- Altered regulation of c-jun and its involvement in anchorage-independent growth of human lung cancers.Oncogene · 2006Article
- p75-Ras-GRF1 is a c-Jun/AP-1 target protein: its up regulation results in increased Ras activity and is necessary for c-Jun-induced nonadherent growth of Rat1a cells.Molecular and cellular biology · 2005Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
cJun is a major component of the transcription factor AP-1 and mediates a diverse set of biologic properties including proliferation, differentiation, and apoptosis. To identify cJun-responsive genes, we inducibly expressed cJun in Rat-1a cells and observed two distinct phenotypes: changes in cellular morphology with adherent growth and anchorage-independent growth. The biologic effects of cJun were entirely reversible demonstrating that they require the continued presence of cJun. To determine the genes, which mediate the biologic effects of cJun, we employed multiple methods including differential gene analysis, suppression subtractive hybridization, and cDNA microarrays. We identified 38 cJun-responsive genes including three uncharacterized genes under adherent and/or nonadherent conditions. Half of the known 36 genes were cytoskeleton- and adhesion-related genes, suggesting a major role of cJun in the regulation of the genes related to cell morphology. As proof of the principle that this approach could identify genes whose upregulation was necessary for nonadherent growth, we investigated one gene, stathmin whose upregulation by cJun was observed only under these conditions. Although overexpression of stathmin did not result in nonadherent growth, inhibition of stathmin protein expression by antisense oligonucleotides in cJun-induced Rat-1a cells prevented nonadherent growth. These results suggest that stathmin plays an essential role in anchorage-independent growth by cJun and may be a potential target for specific inhibitors for AP-1-dependent processes involved in carcinogenesis.
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