ArticleThe Journal of physiology2003
Purinergic and adrenergic Ca2+ transients during neurogenic contractions of rat mesenteric small arteries.
Article in The Journal of physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 57 citations in OpenAlex.
- The Calcium Signaling Mechanisms in Arterial Smooth Muscle and Endothelial Cells.Comprehensive Physiology · 2021Article
- Article
- Aging alters spontaneous and neurotransmitter-mediated CaMicrocirculation (New York, N.Y. : 1994) · 2020Article
- Calcium signals that determine vascular resistance.Wiley interdisciplinary reviews. Systems biology and medicine · 2019Review
- Vasomotion of mice mesenteric arteries during low oxygen levels.European journal of medical research · 2018Article
- Imaging sympathetic neurogenic CaAutonomic neuroscience : basic & clinical · 2017Review
- Mechanisms of Vascular Smooth Muscle Contraction and the Basis for Pharmacologic Treatment of Smooth Muscle Disorders.Pharmacological reviews · 2016Review
- Suppression of peripheral sympathetic activity underlies protease-activated receptor 2-mediated hypotension.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2014Article
- Opposing roles of smooth muscle BK channels and ryanodine receptors in the regulation of nerve-evoked constriction of mesenteric resistance arteries.American journal of physiology. Heart and circulatory physiology · 2014Article
- Calcium dynamics in vascular smooth muscle.Microcirculation (New York, N.Y. : 1994) · 2013Review
- Vascular tone and Ca(2+) signaling in murine cremaster muscle arterioles in vivo.Microcirculation (New York, N.Y. : 1994) · 2013Article
- Perivascular innervation: a multiplicity of roles in vasomotor control and myoendothelial signaling.Microcirculation (New York, N.Y. : 1994) · 2013Review
- Signalling to contractile proteins by muscarinic and purinergic pathways in neurally stimulated bladder smooth muscle.The Journal of physiology · 2012Article
- A possible role of the cholinergic and purinergic receptor interaction in the regulation of the rat urinary bladder function.Journal of muscle research and cell motility · 2012Article
- Sympathetic nerve stimulation induces local endothelial Ca2+ signals to oppose vasoconstriction of mouse mesenteric arteries.American journal of physiology. Heart and circulatory physiology · 2012Article
- Sympathetic overdrive in obesity involves purinergic hyperactivity in the resistance vasculature.The Journal of physiology · 2011Article
- Dynamic monitoring of NET activity in mature murine sympathetic terminals using a fluorescent substrate.British journal of pharmacology · 2010Article
- Nerve-evoked purinergic signalling suppresses action potentials, Ca2+ flashes and contractility evoked by muscarinic receptor activation in mouse urinary bladder smooth muscle.The Journal of physiology · 2009Article
- Sympathetic neurogenic Ca2+ signalling in rat arteries: ATP, noradrenaline and neuropeptide Y.Experimental physiology · 2009Article
- Smooth muscle cell calcium activation mechanisms.The Journal of physiology · 2008Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Contraction of small arteries is regulated by the sympathetic nervous system, but the Ca2+ transients during neurally stimulated contraction of intact small arteries have not yet been recorded. We loaded rat mesenteric small arteries with the fluorescent Ca2+ indicator fluo-4 and mounted them in a myograph that permitted simultaneous (i) high-speed confocal imaging of fluorescence from individual smooth muscle cells, (ii) electrical stimulation of perivascular nerves, and (iii) recording of isometric tension. Sympathetic neuromuscular transmission was achieved by electrical field stimulation (EFS) (frequency, 10 Hz; pulse voltage, 40 V; pulse duration, 0.2 ms) in the presence of capsaicin and scopolamine (to inhibit 'sensory' and cholinergic nerves, respectively). During the first 20 s of EFS, force rose to a small peak and then declined. During this time, junctional Ca2+ transients (jCaTs) were present at relatively high frequency. We have previously attributed jCaTs to influx of Ca2+ through post-junctional P2X receptors activated by ATP. Propagating asynchronous Ca2+ waves, previously associated with bath-applied alpha1-adrenoceptor agonists, were not initially present. During the next 2.5 min of EFS, force rose slowly, and asynchronous propagating Ca2+ waves appeared. The selective alpha1-adrenoceptor antagonist prazosin abolished both the slowly developing contraction and the Ca2+ waves, but reduced the initial transient contraction by only ~25 %. During 3 min of EFS in prazosin, the frequency of jCaTs declined markedly; at sites at which at least one jCaT occurred, the average probability of a jCaT was 0.008 +/- 0.002 pulse-1 in the first 20 s and 0.0007 +/- 0.0002 pulse-1 in the last 20 s. We suggest that (i) ATP released from sympathetic varicosities activates the initial, transient, contraction and the activator Ca2+ is derived largely from jCaTs, and (ii) sympathetically released noradrenaline (NA) activates the later, major contraction through mechanisms involving alpha1-adrenoceptors and which are associated with propagating Ca2+ waves.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.