ArticleThe Journal of biological chemistry2003
RNA-structural mimicry in Escherichia coli ribosomal protein L4-dependent regulation of the S10 operon.
Article in The Journal of biological chemistry, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Extraribosomal Functions of Bacterial Ribosomal Proteins-An Update, 2023.International journal of molecular sciences · 2024Review
- Transcription complexes as RNA chaperones.Transcription · 2021Article
- Rerouting of ribosomal proteins into splicing in plant organelles.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Posttranscriptional Regulation ofJournal of bacteriology · 2020Article
- Diversity, versatility and complexity of bacterial gene regulation mechanisms: opportunities and drawbacks for applications in synthetic biology.FEMS microbiology reviews · 2019Review
- Roles of Transcriptional and Translational Control Mechanisms in Regulation of Ribosomal Protein Synthesis in Escherichia coli.Journal of bacteriology · 2017Article
- Recognizing RNA structural motifs in HT-SELEX data for ribosomal protein S15.BMC bioinformatics · 2017Article
- Article
- Regulation of the rplY gene encoding 5S rRNA binding protein L25 in Escherichia coli and related bacteria.RNA (New York, N.Y.) · 2015Article
- Ribosomal proteins as unrevealed caretakers for cellular stress and genomic instability.Oncotarget · 2014Review
- Identification of candidate structured RNAs in the marine organism 'Candidatus Pelagibacter ubique'.BMC genomics · 2009Article
- How common are extraribosomal functions of ribosomal proteins?Molecular cell · 2009Review
- Ribosomal protein L3 functions as a 'rocker switch' to aid in coordinating of large subunit-associated functions in eukaryotes and Archaea.Nucleic acids research · 2008Article
- A new regulatory circuit in ribosomal protein operons: S2-mediated control of the rpsB-tsf expression in vivo.RNA (New York, N.Y.) · 2008Article
- Ribosomal protein L20 controls expression of the Bacillus subtilis infC operon via a transcription attenuation mechanism.Nucleic acids research · 2007Article
- Predicting cis-acting elements of Lactobacillus plantarum by comparative genomics with different taxonomic subgroups.Nucleic acids research · 2006Article
- Regulation of ribosomal protein synthesis in Vibrio cholerae.Journal of bacteriology · 2004Article
- Specific recognition of rpsO mRNA and 16S rRNA by Escherichia coli ribosomal protein S15 relies on both mimicry and site differentiation.Molecular microbiology · 2004Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Ribosomal protein L4 regulates the 11-gene S10 operon in Escherichia coli by acting, in concert with transcription factor NusA, to cause premature transcription termination at a Rho-independent termination site in the leader sequence. This process presumably involves L4 interaction with the leader mRNA. Here, we report direct, specific, and independent binding of ribosomal protein L4 to the S10 mRNA leader in vitro. Most of the binding energy is contributed by a small hairpin structure within the leader region, but a 64-nucleotide sequence is required for the bona fide interaction. Binding to the S10 leader mRNA is competed by the 23 S rRNA L4 binding site. Although the secondary structures of the mRNA and rRNA binding sites appear different, phosphorothioate footprinting of the L4-RNA complexes reveals close structural similarity in three dimensions. Mutational analysis of the mRNA binding site is compatible with the structural model. In vitro binding of L4 induces structural changes of the S10 leader RNA, providing a first clue for how protein L4 may provoke transcription termination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.