ArticleNucleic acids research2003
Transcription affects formation and processing of intermediates in oligonucleotide-mediated gene alteration.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 62 citations in OpenAlex.
- Editing the Genome Without Double-Stranded DNA Breaks.ACS chemical biology · 2018Review
- Stimulation of oligonucleotide-directed gene correction by Redβ expression and MSH2 depletion in human HT1080 cells.Molecules and cells · 2015Article
- Emerging gene editing strategies for Duchenne muscular dystrophy targeting stem cells.Frontiers in physiology · 2014Review
- Strand bias influences the mechanism of gene editing directed by single-stranded DNA oligonucleotides.Nucleic acids research · 2011Article
- Progress and prospects: oligonucleotide-directed gene modification in mouse embryonic stem cells: a route to therapeutic application.Gene therapy · 2011Review
- Parameters of oligonucleotide-mediated gene modification in mouse ES cells.Journal of cellular and molecular medicine · 2010Article
- DNA breakage associated with targeted gene alteration directed by DNA oligonucleotides.Mutation research · 2009Article
- Targeted correction of a thalassemia-associated beta-globin mutation induced by pseudo-complementary peptide nucleic acids.Nucleic acids research · 2009Article
- A comparison of synthetic oligodeoxynucleotides, DNA fragments and AAV-1 for targeted episomal and chromosomal gene repair.BMC biotechnology · 2009Article
- Site-specific gene modification by oligodeoxynucleotides in mouse bone marrow-derived mesenchymal stem cells.Gene therapy · 2008Article
- Oligonucleotide-mediated gene targeting in human hepatocytes: implications of mismatch repair.Oligonucleotides · 2008Article
- Manipulation of cell cycle progression can counteract the apparent loss of correction frequency following oligonucleotide-directed gene repair.BMC molecular biology · 2007Article
- Delivery and mechanistic considerations for the production of knock-in mice by single-stranded oligonucleotide gene targeting.Gene therapy · 2007Article
- Oligonucleotide-directed gene repair in wheat using a transient plasmid gene repair assay system.Plant cell reports · 2006Article
- Effective oligonucleotide-mediated gene disruption in ES cells lacking the mismatch repair protein MSH3.Gene therapy · 2006Article
- Differential cellular responses to exogenous DNA in mammalian cells and its effect on oligonucleotide-directed gene modification.Gene therapy · 2006Article
- Generation of a mouse mutant by oligonucleotide-mediated gene modification in ES cells.Nucleic acids research · 2006Article
- Site-specific base changes in the coding or promoter region of the human beta- and gamma-globin genes by single-stranded oligonucleotides.The Biochemical journal · 2005Article
- Gene therapy progress and prospects: targeted gene repair.Gene therapy · 2005Review
- Reaction parameters of targeted gene repair in mammalian cells.Molecular biotechnology · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The role of transcription in oligonucleotide (ODN)-directed gene modification has been investigated in mammalian cells. The importance of transcription is demonstrated using mammalian cell lines with varying degrees of transcription of the mutant LacZ reporter gene, residing in both episome and chromosome. Gene correction occurs more efficiently when the target gene is actively transcribed and antisense ODN is more active than sense ODN. Using an approach that combines biochemical studies with a cell-based assay to measure the functional activity of intermediates it is shown that a joint molecule, consisting of supercoiled DNA and homologous ODN targeted to correct the mutated base, is a functional intermediate in the gene repair process. Furthermore, this approach showed that a resected joint molecule is a downstream intermediate of the D-loop. These results indicate that the primary reason for efficient gene repair exhibited by the antisense ODN is its increased accessibility to the non-transcribed strand, and as a consequence an increased formation of intermediate during active transcription. Moreover, the processing of intermediates was also affected by transcription, suggesting that ODN-directed gene repair may be linked to transcription-coupled repair. Thus, transcription plays an important role in ODN-directed gene repair by affecting the formation and processing of key intermediates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.