ArticleNucleic acids research2003
Incorporation of reporter molecule-labeled nucleotides by DNA polymerases. II. High-density labeling of natural DNA.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Synthesizing supercoiled circular DNA molecules in vitro.Nucleic acids research · 2025Article
- Generation of densely labeled oligonucleotides for the detection of small genomic elements.Cell reports methods · 2024Article
- High-yield ligation-free assembly of DNA constructs with nucleosome positioning sequence repeats for single-molecule manipulation assays.The Journal of biological chemistry · 2023Article
- Incorporation ofInternational journal of molecular sciences · 2023Article
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- Mononucleotide repeat expansions with non-natural polymerase substrates.Scientific reports · 2021Article
- Rolling Circle Amplification as a Universal Method for the Analysis of a Wide Range of Biological Targets.Russian journal of bioorganic chemistry · 2021Article
- DNA bridges: A novel platform for single-molecule sequencing and other DNA-protein interaction applications.PloS one · 2021Article
- FISH Going Meso-Scale: A Microscopic Search for Chromatin Domains.Frontiers in cell and developmental biology · 2021Review
- In-gel fluorescence detection by DNA polymerase elongation.APL bioengineering · 2020Article
- Synthetic Elaboration of Native DNA by RASS (SENDR).ACS central science · 2020Article
- dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase.Nucleic acids research · 2018Article
- In vitro synthesis of gene-length single-stranded DNA.Scientific reports · 2018Article
- Comparative Study of Novel Fluorescent Cyanine Nucleotides: Hybridization Analysis of Labeled PCR Products Using a Biochip.Journal of fluorescence · 2017Article
- Distributed biotin-streptavidin transcription roadblocks for mapping cotranscriptional RNA folding.Nucleic acids research · 2017Article
- Fluorescence Turn-On Sensing of DNA Duplex Formation by a Tricyclic Cytidine Analogue.Journal of the American Chemical Society · 2017Article
- Article
- Efficient enzymatic synthesis and dual-colour fluorescent labelling of DNA probes using long chain azido-dUTP and BCN dyes.Nucleic acids research · 2016Article
- The Toolbox for Modified Aptamers.Molecular biotechnology · 2016Review
- Artificial specific binders directly recovered from chemically modified nucleic acid libraries.Journal of nucleic acids · 2012Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The modification of nucleic acids using nucleotides linked to detectable reporter or functional groups is an important experimental tool in modern molecular biology. This enhances DNA or RNA detection as well as expanding the catalytic repertoire of nucleic acids. Here we present the evaluation of a broad range of modified deoxyribonucleoside 5'-triphosphates (dNTPs) covering all four naturally occurring nucleobases for potential use in DNA modification. A total of 30 modified dNTPs with either fluorescent or non-fluorescent reporter group attachments were systematically evaluated individually and in combinations for high-density incorporation using different model and natural DNA templates. Furthermore, we show a side-by-side comparison of the incorporation efficiencies of a family A (Taq) and B (Vent(R) exo-) type DNA polymerase using the differently modified dNTP substrates. Our results show superior performance by a family B-type DNA polymerase, Vent(R) exo-, which is able to fully synthesize a 300 bp DNA product when all natural dNTPs are completely replaced by their biotin-labeled dNTP analogs. Moreover, we present systematic testing of various combinations of fluorescent dye-modified dNTPs enabling the simultaneous labeling of DNA with up to four differently modified dNTPs.
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