ArticleNucleic acids research2003
Spectroscopic studies of the multiple binding modes of a trimethine-bridged cyanine dye with DNA.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 74 citations in OpenAlex.
- Single-Photon DNA Photocleavage up to 905 nm by a Benzylated 4-Quinolinium Carbocyanine Dye.ACS omega · 2025Article
- DNA Framework-Engineered Assembly of Cyanine Dyes for Structural Identification of Nucleic Acids.JACS Au · 2024Article
- Conjugated Oligoelectrolyte with DNA Affinity for Enhanced Nuclear Imaging and Precise DNA Quantification.Biosensors · 2024Article
- Trimethine Cyanine Dyes as NA-Sensitive Probes for Visualization of Cell Compartments in Fluorescence Microscopy.ACS omega · 2022Article
- Photonics of Trimethine Cyanine Dyes as Probes for Biomolecules.Molecules (Basel, Switzerland) · 2022Review
- Article
- Programming Structured DNA Assemblies to Probe Biophysical Processes.Annual review of biophysics · 2019Review
- Combination probes with intercalating anchors and proximal fluorophores for DNA and RNA detection.Nucleic acids research · 2016Article
- Characterization of the DNA-Mediated Oxidation of Dps, A Bacterial Ferritin.Journal of the American Chemical Society · 2016Article
- Symmetric Meso-Chloro-Substituted Pentamethine Cyanine Dyes Containing Benzothiazolyl/Benzoselenazolyl Chromophores Novel Synthetic Approach and Studies on Photophysical Properties upon Interaction with bio-Objects.Journal of fluorescence · 2016Article
- Twisted cyanines: a non-planar fluorogenic dye with superior photostability and its use in a protein-based fluoromodule.Journal of the American Chemical Society · 2013Article
- The mechanism of benzothiazole styrylcyanine dyes binding with dsDNA: studies by spectral-luminescent methods.Journal of fluorescence · 2008Article
- Fluorescent DNA nanotags: supramolecular fluorescent labels based on intercalating dye arrays assembled on nanostructured DNA templates.Journal of the American Chemical Society · 2007Article
- Hydration changes accompanying the binding of minor groove ligands with DNA.Biophysical journal · 2007Article
- Hydration changes in the association of Hoechst 33258 with DNA.Biochemistry · 2005Article
- Fluorescent properties of pentamethine cyanine dyes with cyclopentene and cyclohexene group in presence of biological molecules.Journal of fluorescence · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The interaction between DNA and a benzothiazole-quinoline cyanine dye with a trimethine bridge (TO-PRO-3) results in the formation of three noncovalent complexes. Unbound TO-PRO-3 has an absorption maximum (lambda(max)) of 632 nm, while the bound dyes (with calf thymus DNA) have electronic transitions with lambda(max) = 514 nm (complex I), 584 nm (complex II) and 642 nm (complex III). The blue shifts in the electronic transitions and the bisignate shape of the circular dichroism bands indicate that TO-PRO-3 aggregates with DNA. Complex I has a high dye:base pair stoichiometry, which does not depend on base sequence or base modifications. The bound dyes exhibit strong interdye coupling, based on studies with a short oligonucleotide and on enhanced resonance scattering. From thermal dissociation studies, the complex is weakly associated with DNA. Studies with poly(dGdC)2 and poly(dIdC)2 and competitive binding with distamycin demonstrate that complex II is bound in the minor groove. This complex stabilizes the helix against dissociation. For complex III, the slightly red-shifted electronic transition and the stoichiometry are most consistent with intercalation. Using poly(dAdT)2, the complexes have the following dye mole fractions (X(dye)): X(dye) = 0.65 (complex I), 0.425 (complex II) and 0.34 (complex III).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.