ArticleNucleic acids research2003
RNomics in Drosophila melanogaster: identification of 66 candidates for novel non-messenger RNAs.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 41 papers.
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41 citing papers in PubMed, 86 citations in OpenAlex.
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- Identification and functional characterization of intermediate-size non-coding RNAs in maize.BMC genomics · 2018Article
- Deletion of Drosophila Nopp140 induces subcellular ribosomopathies.Chromosoma · 2015Article
- Computational prediction and validation of C/D, H/ACA and Eh_U3 snoRNAs of Entamoeba histolytica.BMC genomics · 2012Article
- Experimental identification and analysis of macronuclear non-coding RNAs from the ciliate Tetrahymena thermophila.Nucleic acids research · 2012Article
- Clustered organization, polycistronic transcription, and evolution of modification-guide snoRNA genes in Euglena gracilis.Molecular genetics and genomics : MGG · 2012Article
- The little elongation complex regulates small nuclear RNA transcription.Molecular cell · 2011Article
- Experimental RNomics and genomic comparative analysis reveal a large group of species-specific small non-message RNAs in the silkworm Bombyx mori.Nucleic acids research · 2011Article
- Identification and functional characterization of small non-coding RNAs in Xanthomonas oryzae pathovar oryzae.BMC genomics · 2011Article
- Post-transcriptional modification of RNAs by artificial Box H/ACA and Box C/D RNPs.Methods in molecular biology (Clifton, N.J.) · 2011Article
- Computational prediction of Caenorhabditis box H/ACA snoRNAs using genomic properties of their host genes.RNA (New York, N.Y.) · 2010Article
- Identification of novel non-coding RNAs using profiles of short sequence reads from next generation sequencing data.BMC genomics · 2010Article
- Systematic identification and evolutionary features of rhesus monkey small nucleolar RNAs.BMC genomics · 2010Article
- Small Cajal body-specific RNAs of Drosophila function in the absence of Cajal bodies.Molecular biology of the cell · 2009Article
- Systematic identification and characterization of chicken (Gallus gallus) ncRNAs.Nucleic acids research · 2009Article
- A conserved WD40 protein binds the Cajal body localization signal of scaRNP particles.Molecular cell · 2009Article
- Experimental discovery of sRNAs in Vibrio cholerae by direct cloning, 5S/tRNA depletion and parallel sequencing.Nucleic acids research · 2009Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
By generating a specialised cDNA library from four different developmental stages of Drosophila melanogaster, we have identified 66 candidates for small non-messenger RNAs (snmRNAs) and have confirmed their expression by northern blot analysis. Thirteen of them were expressed at certain stages of D.melanogaster development, only. Thirty-five species belong to the class of small nucleolar RNAs (snoRNAs), divided into 15 members from the C/D subclass and 20 members from the H/ACA subclass, which mostly guide 2'-O-methylation and pseudouridylation, respectively, of rRNA and snRNAs. These also include two outstanding C/D snoRNAs, U3 and U14, both functioning as pre-rRNA chaperones. Surprisingly, the sequence of the Drosophila U14 snoRNA reflects a major change of function of this snoRNA in Diptera relative to yeast and vertebrates. Among the 22 snmRNAs lacking known sequence and structure motifs, five were located in intergenic regions, two in introns, five in untranslated regions of mRNAs, eight were derived from open reading frames, and two were transcribed opposite to an intron. Interestingly, detection of two RNA species from this group implies that certain snmRNA species are processed from alternatively spliced pre-mRNAs. Surprisingly, a few snmRNA sequences could not be found on the published D.melanogaster genome, which might suggest that more snmRNA genes (as well as mRNAs) are hidden in unsequenced regions of the genome.
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