ArticleBiological procedures online
A semi-quantitative RT-PCR method to measure the in vivo effect of dietary conjugated linoleic acid on porcine muscle PPAR gene expression.
Article in Biological procedures online. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 70 citations in OpenAlex.
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- Bamboo shoot dietary fiber alleviates gut microbiota dysbiosis and modulates liver fatty acid metabolism in mice with high-fat diet-induced obesity.Frontiers in nutrition · 2023Article
- Exon Skipping Through Chimeric AntisenseNucleic acid therapeutics · 2022Article
- Conjugated linoleic acid regulates adipocyte fatty acid binding protein expressionFrontiers in nutrition · 2022Article
- Evaluating the ameliorative efficacy of Spirulina platensis on spermatogenesis and steroidogenesis in cadmium-intoxicated rats.Environmental science and pollution research international · 2016Article
- MicroRNA-10b and minichromosome maintenance complex component 5 gene as prognostic biomarkers in breast cancer.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2015Article
- Production of Cloned Miniature Pigs Expressing High Levels of Human Apolipoprotein(a) in Plasma.PloS one · 2015Article
- Expression of bioactive porcine interferon-alpha in Lactobacillus casei.World journal of microbiology & biotechnology · 2014Article
- Quercetin and omega 3 ameliorate oxidative stress induced by aluminium chloride in the brain.Journal of molecular neuroscience : MN · 2014Article
- Evaluation of urinary HURP mRNA as a marker for detection of bladder cancer: relation to bilharziasis.Medical oncology (Northwood, London, England) · 2014Article
- Conjugated linoleic acid isomers and their precursor fatty acids regulate peroxisome proliferator-activated receptor subtypes and major peroxisome proliferator responsive element-bearing target genes in HepG2 cell model.Journal of Zhejiang University. Science. B · 2013Article
- Human papillomavirus-16 (HPV-16) infection association with CIAP-2 expression in head and neck cancer.Medical oncology (Northwood, London, England) · 2012Article
- Detection of hyaluronidase RNA and activity in urine of schistosomal and non-schistosomal bladder cancer.Medical oncology (Northwood, London, England) · 2012Article
- Triacylglycerol synthesis by PDAT1 in the absence of DGAT1 activity is dependent on re-acylation of LPC by LPCAT2.BMC plant biology · 2012Article
- The expression of tubulin cofactor A (TBCA) is regulated by a noncoding antisense Tbca RNA during testis maturation.PloS one · 2012Article
- Attenuated Salmonella typhimurium carrying shRNA-expressing vectors elicit RNA interference in murine bladder tumors.Acta pharmacologica Sinica · 2011Article
- Molecular characterization and association analysis of FBXO40 with partial hematological indexes in pig.Molecular biology reports · 2010Article
- Identification of genes related to the development of bamboo rhizome bud.Journal of experimental botany · 2010Article
- Comparing subcutaneous adipose tissue in beef and muskox with emphasis on trans 18:1 and conjugated linoleic acids.Lipids · 2007Article
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conjugated linoleic acid (CLA) can activate (in vitro) the nuclear transcription factors known as the peroxisome proliferators activated receptors (PPAR). CLA was fed at 11 g CLA/kg of feed for 45d to castrated male pigs (barrows) to better understand long term effects of PPAR activation in vivo. The barrows fed CLA had lean muscle increased by 3.5% and overall fat reduced by 9.2% but intramuscular fat (IMF %) was increased by 14% (P < 0.05). To measure the effect of long term feeding of CLA on porcine muscle gene expression, a semi-quantitative RT-PCR method was developed using cDNA normalized against the housekeeping genes cyclophilin and beta-actin. This method does not require radioactivity or expensive PCR instruments with real-time fluorescent detection. PPARgamma and the PPAR responsive gene AFABP but not PPARalpha were significantly increased (P < 0.05) in the CLA fed pig's muscle. PPARalpha and PPARgamma were also quantitatively tested for large differences in gene expression by western blot analysis but no significant difference was detected at this level. Although large differences in gene expression of the PPAR transcriptional factors could not be confirmed by western blotting techniques. The increased expression of AFABP gene, which is responsive to PPAR transcriptional factors, confirmed that dietary CLA can induce a detectable increase in basal PPAR transcriptional activity in the live animal.
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Registered trials
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