ArticleJournal of clinical microbiology2003
Identification and characterization of a new variant of Shiga toxin 1 in Escherichia coli ONT:H19 of bovine origin.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 100 citations in OpenAlex.
- Excess A-subunits of Shiga toxin 2a are produced in enterohemorrhagic Escherichia coli.Scientific reports · 2025Article
- A Comprehensive Review on Shiga Toxin Subtypes and Their Niche-Related Distribution Characteristics in Shiga-Toxin-ProducingMicroorganisms · 2024Review
- Identification and Characterization of tenMicroorganisms · 2023Article
- Detection and analysis of Shiga toxin producing and enteropathogenic Escherichia coli in cattle from Tierra del Fuego, Argentina.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Phytosynthesis of Silver Nanoparticles UsingMaterials (Basel, Switzerland) · 2023Article
- Molecular Biology ofToxins · 2020Review
- Isolation of Shiga toxin-producingVeterinary world · 2018Article
- Novel Antibiotic Testing Approaches Reveal Reduced Antibiotic Efficacy Against Shiga Toxin-ProducingFrontiers in microbiology · 2018Article
- Degradation and inactivation of Shiga toxins by nitrogen gas plasma.AMB Express · 2017Article
- Divergent Evolution of the repFII Replicon of IncF Plasmids Carrying Cytotoxic Necrotizing Factor cnf2, Cytolethal Distending Toxin cdtIII, and f17Ae Fimbrial Variant Genes in Type 2 Necrotoxigenic Escherichia coli Isolates from Calves.Applied and environmental microbiology · 2016Article
- A globotetraosylceramide (Gb₄) receptor-based ELISA for quantitative detection of Shiga toxin 2e.The Journal of veterinary medical science · 2015Article
- Identification and detection of three new F17 fimbrial variants in Escherichia coli strains isolated from cattle.Veterinary research · 2014Article
- Shiga Toxin (Stx) Classification, Structure, and Function.Microbiology spectrum · 2014Review
- Isolation and identification of an Enterobacter cloacae strain producing a novel subtype of Shiga toxin type 1.Journal of clinical microbiology · 2014Article
- New high-affinity monoclonal antibodies against Shiga toxin 1 facilitate the detection of hybrid Stx1/Stx2 in vivo.PloS one · 2014Article
- Virulence profiling of Shiga toxin-producing Escherichia coli recovered from domestic farm animals in Northwestern Mexico.Frontiers in cellular and infection microbiology · 2014Article
- Shiga toxin-binding site for host cell receptor GPP130 reveals unexpected divergence in toxin-trafficking mechanisms.Molecular biology of the cell · 2013Article
- Detection and characterization of Shiga toxin-producing Escherichia coli in faeces and lymphatic tissue of free-ranging deer.Epidemiology and infection · 2013Article
- Multicenter evaluation of a sequence-based protocol for subtyping Shiga toxins and standardizing Stx nomenclature.Journal of clinical microbiology · 2012Article
- Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new variant of Shiga toxin 1 (Stx1), designated Stx1d, which deviates considerably more than any other known variant from Stx1 encoded by phage 933J, was identified in an Escherichia coli strain, ONT:H19, isolated from bovine feces. The complete stx(1) gene of this strain was amplified and sequenced. Nucleotide sequence homology with stx(1) from phage 933J was only 91%, resulting in the substitution of 20 amino acids in the A subunit and 7 amino acids in the B subunit of the protein. Cell culture supernatant of this strain, which was negative for stx(2) by PCR testing, was cytotoxic to Vero cells and gave positive results in two commercial enzyme-linked immunosorbent assays for Stx. PCR primers were constructed for the specific detection of the new variant. The findings of this study suggest that Stx1 is not as conserved as thought before and that there might be more variants which cannot be detected by commonly used PCR methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.