ArticleJournal of clinical microbiology2003
Molecular epidemiology of Mycobacterium avium subsp. paratuberculosis: evidence for limited strain diversity, strain sharing, and identification of unique targets for diagnosis.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 2 syntheses or guidelines pooled it, 97 citations in OpenAlex.
- The zoonotic potential of Mycobacterium avium ssp. paratuberculosis: a systematic review and meta-analyses of the evidence.Epidemiology and infection · 2015Pooled it
- The zoonotic potential of Mycobacterium avium spp. paratuberculosis: a systematic review.Canadian journal of public health = Revue canadienne de sante publiquePooled it
- Current perspectives on avian tuberculosis in domestic and wild birds: Molecular diagnostics, genomic surveillance, and vaccination challenges.Open veterinary journal · 2026Review
- Genotyping methods and molecular epidemiology ofInternational journal of veterinary science and medicine · 2018Review
- Draft Genome Sequence of a Rare PigmentedGenome announcements · 2017Article
- Novel Single Nucleotide Polymorphism-Based Assay for Genotyping Mycobacterium avium subsp. paratuberculosis.Journal of clinical microbiology · 2016Article
- Comparative Evaluation of Different Test Combinations for Diagnosis of Mycobacterium avium Subspecies paratuberculosis Infecting Dairy Herds in India.BioMed research international · 2015Article
- Improved short-sequence-repeat genotyping of Mycobacterium avium subsp. paratuberculosis by using matrix-assisted laser desorption ionization-time of flight mass spectrometry.Applied and environmental microbiology · 2014Article
- Mycobacterium avium subspecies paratuberculosis: an insidious problem for the ruminant industry.Tropical animal health and production · 2013Review
- Isolation of Mycobacterium avium subspecies paratuberculosis from Ugandan cattle and strain differentiation using optimised DNA typing techniques.BMC veterinary research · 2012Article
- Fecal detection of Mycobacterium avium paratuberculosis using the IS900 DNA sequence in Crohn's disease and ulcerative colitis patients and healthy subjects.Digestive diseases and sciences · 2011Article
- Amplified fragment length polymorphism reveals specific epigenetic distinctions between Mycobacterium avium subspecies paratuberculosis isolates of various isolation types.Journal of clinical microbiology · 2011Article
- Exploring the zoonotic potential of Mycobacterium avium subspecies paratuberculosis through comparative genomics.PloS one · 2011Article
- Detection of Mycobacterium avium subsp. paratuberculosis by a Direct In Situ PCR Method.Veterinary medicine international · 2011Article
- Efficient differentiation of Mycobacterium avium complex species and subspecies by use of five-target multiplex PCR.Journal of clinical microbiology · 2010Article
- Detection of Mycobacterium avium subsp. paratuberculosis in drinking water and biofilms by quantitative PCR.Applied and environmental microbiology · 2010Article
- Iron-sparing response of Mycobacterium avium subsp. paratuberculosis is strain dependent.BMC microbiology · 2010Article
- Multiplex nested PCR for detection of Xanthomonas axonopodis pv. allii from onion seeds.Applied and environmental microbiology · 2010Article
- Article
- Comparative analysis of Mycobacterium avium subsp. paratuberculosis isolates from cattle, sheep and goats by short sequence repeat and pulsed-field gel electrophoresis typing.BMC microbiology · 2008Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The objectives of this study were to understand the molecular diversity of animal and human strains of Mycobacterium avium subsp. paratuberculosis isolated in the United States and to identify M. avium subsp. paratuberculosis-specific diagnostic molecular markers to aid in disease detection, prevention, and control. Multiplex PCR of IS900 integration loci (MPIL) and amplified fragment length polymorphism (AFLP) analyses were used to fingerprint M. avium subsp. paratuberculosis isolates recovered from animals (n = 203) and patients with Crohn's disease (n = 7) from diverse geographic localities. Six hundred bacterial cultures, including M. avium subsp. paratuberculosis (n = 303), non-M. avium subsp. paratuberculosis mycobacteria (n = 129), and other nonmycobacterial species (n = 168), were analyzed to evaluate the specificity of two IS900 integration loci and a newly described M. avium subsp. paratuberculosis-specific sequence (locus 251) as potential targets for the diagnosis of M. avium subsp. paratuberculosis. MPIL fingerprint analysis revealed that 78% of bovine origin M. avium subsp. paratuberculosis isolates clustered together into a major node, whereas isolates from human and ovine sources showed greater genetic diversity. MPIL analysis also showed that the M. avium subsp. paratuberculosis isolates from ovine and bovine sources from the same state were more closely associated than were isolates from different geographic regions, suggesting that some of the strains are shared between these ruminant species. AFLP fingerprinting revealed a similar pattern, with most isolates from bovine sources clustering into two major nodes, while those recovered from sheep or humans were clustered on distinct branches. Overall, this study identified a high degree of genetic similarity between M. avium subsp. paratuberculosis strains recovered from cows regardless of geographic origin. Further, the results of our analyses reveal a relatively higher degree of genetic heterogeneity among M. avium subsp. paratuberculosis isolates recovered from human and ovine sources.
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Registered trials
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