ArticleJournal of clinical microbiology2003
Evaluation of pulsed-field gel electrophoresis as a tool for determining the degree of genetic relatedness between strains of Escherichia coli O157:H7.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed.
- Genomic insights into the genetic diversity, resistance determinants, and plasmid content of carbapenem-resistant Acinetobacter baumannii clinical isolates.Archives of microbiology · 2026Article
- The synergy ofThe Pan African medical journal · 2025Article
- Virulence genes and pulsed-field gel electrophoresis profiles of Shiga toxin-producingIranian journal of microbiology · 2024Article
- Clonal spread of multi-resistant Gallibacterium anatis isolates among Iranian broilers and layers.Veterinary research · 2021Article
- SupershedInternational journal of microbiology · 2020Article
- Molecular typing of cytotoxin-producingNew microbes and new infections · 2019Article
- Comparative genomics reveals structural and functional features specific to the genome of a foodborne Escherichia coli O157:H7.BMC genomics · 2019Article
- Laboratory Methods in Molecular Epidemiology: Bacterial Infections.Microbiology spectrum · 2018Review
- Antimicrobial resistance of Enterococcus faecium strains isolated from commercial probiotic products used in cattle and swine.Journal of animal science · 2018Article
- Whole Genome Sequencing for Genomics-Guided Investigations of Escherichia coli O157:H7 Outbreaks.Frontiers in microbiology · 2016Article
- Next-Generation Sequencing and Comparative Analysis of Sequential Outbreaks Caused by Multidrug-Resistant Acinetobacter baumannii at a Large Academic Burn Center.Antimicrobial agents and chemotherapy · 2015Article
- Application of whole-genome sequencing for bacterial strain typing in molecular epidemiology.Journal of clinical microbiology · 2015Article
- Geographic divergence of bovine and human Shiga toxin–producing Escherichia coli O157:H7 genotypes, New Zealand.Emerging infectious diseases · 2014Article
- Whole-genome sequencing for high-resolution investigation of methicillin-resistant Staphylococcus aureus epidemiology and genome plasticity.Journal of clinical microbiology · 2014Article
- Precise dissection of an Escherichia coli O157:H7 outbreak by single nucleotide polymorphism analysis.Journal of clinical microbiology · 2013Article
- Characterization of Escherichia coli O157:H7 strains isolated from supershedding cattle.Applied and environmental microbiology · 2013Article
- Identification and characterization of spontaneous deletions within the Sp11-Sp12 prophage region of Escherichia coli O157:H7 Sakai.Applied and environmental microbiology · 2013Article
- Polymorphic Amplified Typing Sequences (PATS) Strain Typing System Accurately Discriminates a Set of Temporally and Spatially Disparate Escherichia coli O157 Isolates Associated with Human Infection.The open microbiology journal · 2013Article
- Phylogeny of Shiga toxin-producing Escherichia coli O157 isolated from cattle and clinically ill humans.Molecular biology and evolution · 2012Article
- High resolution clustering of Salmonella enterica serovar Montevideo strains using a next-generation sequencing approach.BMC genomics · 2012Article
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4 authors.
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Abstract
Pulsed-field gel electrophoresis (PFGE) has been used extensively to investigate the epidemiology of Escherichia coli O157:H7, although it has not been evaluated as a tool for establishing genetic relationships. This is a critical issue when molecular genetic data are used to make inferences about pathogen dissemination. To evaluate this further, genomic DNAs from 62 isolates of E. coli O157:H7 from different cattle herds were digested with XbaI and BlnI and subjected to PFGE. The correlation between the similarity coefficients for these two enzymes was only 0.53. Four additional restriction enzymes (NheI, PacI, SfiI, and SpeI) were used with DNAs from a subset of 14 isolates. The average correlations between similarity coefficients using sets of one, two, and three enzymes were 0.405, 0.568, and 0.648, respectively. Probing with lambda DNA demonstrated that some DNA fragments migrated equal distances in the gel but were composed of nonhomologous genetic material. Genome sequence data from EDL933 indicated that 40 PFGE fragments would be expected from complete XbaI digestion, yet only 19 distinguishable fragments were visible. Two reasons that similarity coefficients from single-enzyme PFGE are poor measures of relatedness (and hence are poorly correlated with other enzymes) are evident from this study: (i) matching bands do not always represent homologous genetic material and (ii) there are limitations to the power of PFGE to resolve bands of nearly identical size. The findings of the present study indicate that if genetic relationships must be inferred in the absence of epidemiologic data, six or more restriction enzymes would be needed to provide a reasonable estimate using PFGE.
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