ArticleJournal of clinical microbiology2003
Characterization of a tandem repeat polymorphism in Legionella pneumophila and its use for genotyping.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 66 citations in OpenAlex.
- Antimicrobial agent susceptibilities of Legionella pneumophila MLVA-8 genotypes.Scientific reports · 2019Article
- Laboratory Methods in Molecular Epidemiology: Bacterial Infections.Microbiology spectrum · 2018Review
- Virulence Traits of Environmental and Clinical Legionella pneumophila Multilocus Variable-Number Tandem-Repeat Analysis (MLVA) Genotypes.Applied and environmental microbiology · 2018Article
- Temperature-Dependent Growth Modeling of Environmental and Clinical Legionella pneumophila Multilocus Variable-Number Tandem-Repeat Analysis (MLVA) Genotypes.Applied and environmental microbiology · 2017Article
- Culture Strategies for Isolation of FastidiousFrontiers in microbiology · 2017Article
- Characterization of Leptospira interrogans Serovars by Polymorphism Variable Number Tandem Repeat Analysis.Jundishapur journal of microbiology · 2015Article
- Contamination of Hospital Water Supplies in Gilan, Iran, with Legionella pneumophila, Escherichia coli, and Pseudomonas aeruginosa.Interdisciplinary perspectives on infectious diseases · 2015Article
- Detection of virulence factors and molecular typing of pathogenic Leptospira from capybara (Hydrochaeris hydrochaeris).Current microbiology · 2012Article
- Multiple-locus variable-number tandem-repeat analysis of pathogenic Yersinia enterocolitica in China.PloS one · 2012Article
- High-throughput typing method to identify a non-outbreak-involved Legionella pneumophila strain colonizing the entire water supply system in the town of Rennes, France.Applied and environmental microbiology · 2011Article
- Multilocus variable number tandem repeat analysis for Salmonella enterica subspecies.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2011Review
- High-resolution in situ genotyping of Legionella pneumophila populations in drinking water by multiple-locus variable-number tandem-repeat analysis using environmental DNA.Applied and environmental microbiology · 2010Article
- Evaluation of a nested-PCR-derived sequence-based typing method applied directly to respiratory samples from patients with Legionnaires' disease.Journal of clinical microbiology · 2009Article
- Article
- Intragenic tandem repeat variation between Legionella pneumophila strains.BMC microbiology · 2008Article
- VNTR analysis reveals unexpected genetic diversity within Mycoplasma agalactiae, the main causative agent of contagious agalactia.BMC microbiology · 2008Article
- Evaluation and strategy for use of MIRU-VNTRplus, a multifunctional database for online analysis of genotyping data and phylogenetic identification of Mycobacterium tuberculosis complex isolates.Journal of clinical microbiology · 2008Article
- Selection and validation of a multilocus variable-number tandem-repeat analysis panel for typing Shigella spp.Journal of clinical microbiology · 2008Article
- Identification of variable-number tandem-repeat (VNTR) sequences in Legionella pneumophila and development of an optimized multiple-locus VNTR analysis typing scheme.Journal of clinical microbiology · 2007Article
- Molecular characterization of Coxiella burnetii isolates by infrequent restriction site-PCR and MLVA typing.BMC microbiology · 2006Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have analyzed the variability of minisatellite sequences (also called variable-number tandem repeats [VNTRs]) in the genome of Legionella pneumophila. Based upon the genome sequence of the Philadelphia-1 strain (serogroup 1), 25 minisatellites were selected and their polymorphisms were analyzed by PCR with the DNA of serogroup 1 to 14 reference strains. For 22 markers, a PCR product of the expected size was found with the DNA of the Philadelphia-1 strain. Most of these markers did not amplify the DNA of other Legionella species or other bacteria used as controls. A polymorphism was observed for seven markers among the L. pneumophila strains tested. To check whether these markers could be used to compare strains of L. pneumophila, we analyzed two groups of isolates from clinical and environmental samples which had been independently genotyped by other methods. The results showed that, for the isolates in these two sets of samples, VNTR typing is as informative as pulsed-field gel electrophoresis for comparison of strains. Sequencing of one minisatellite from 14 reference strains was performed. Comparison of the sequences allowed a classification and confirmed the existence of subspecies of L. pneumophila. We also tested the usefulness of one very polymorphic marker as a tool for the rapid screening of colonies grown from water samples. This allowed the rapid identification of the L. pneumophila colonies and gave a first hint as to the presence of several strains in a single sample.
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