Evidence map›Paper›PMID 12733005›Full record

ArticleImmunogenetics2003

Molecular evolution of V(H)9 germline genes isolated from DBA, BALB, 129 and C57BL mouse strains and sublines.

Paula Zylstra, Andrew Franklin, Karl A Hassan, Kim L Powell, Edward J Steele, Robert V Blanden

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Article in Immunogenetics, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Paula ZylstraDepartment of Biological Sciences, The University of Wollongong, Northfields Avenue, NSW 2522, Wollongong, Australia.
Andrew FranklinDivision of Immunology and Genetics, The John Curtin School of Medical Research, Australian National University, PO Box 334, ACT 2601, Canberra, Australia.
Karl A HassanDepartment of Biological Sciences, The University of Wollongong, Northfields Avenue, NSW 2522, Wollongong, Australia.
Kim L PowellDivision of Immunology and Genetics, The John Curtin School of Medical Research, Australian National University, PO Box 334, ACT 2601, Canberra, Australia.
Edward J SteeleDepartment of Biological Sciences, The University of Wollongong, Northfields Avenue, NSW 2522, Wollongong, Australia.
Robert V BlandenDivision of Immunology and Genetics, The John Curtin School of Medical Research, Australian National University, PO Box 334, ACT 2601, Canberra, Australia. Robert.Blanden@anu.edu.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We have used the polymerase chain reaction (PCR) in an attempt to clone and sequence the exons and hitherto unavailable contiguous flanks of all members of the small V(H) 9 germline gene family from inbred mouse strains and sublines that have had a common ancestry within the last century, and to analyze the molecular evolution of these sequences. Fifteen genuine germline genes were isolated (designated V(H) 9.1 through V(H) 9.15) from strains and sublines of DBA, BALB, 129 and C57BL inbred mice. Of the 15 genuine isolates, nine are novel: seven sequences from DBA strains and sublines ( V(H) 9.3 to V(H) 9.9) and two sequences from C57BL strains ( V(H) 9.13 and V(H) 9.14). We have identified sequencing errors and PCR recombinant artefacts in previously published sequences. We detected no sequence divergence of individual genes shared by the strains and sublines studied. However, we isolated two genes from DBA strains and sublines, V(H) 9.1 and V(H) 9.3, that differ only by five nucleotides encoding three amino acid changes that are concentrated within a 33 nucleotide (11 codon) region. Of these 11 codons, eight encode a putative antigen binding site. There were no differences in the remaining 733 nucleotides sequenced (including both 5' and 3' flanking regions). Potential explanations for the generation of V(H) 9.1 and V(H) 9.3 are discussed.

Indexed as

Evolution, MolecularAnimalsBase SequenceImmunoglobulin Variable RegionMiceMice, Inbred BALB CMice, Inbred C57BLMice, Inbred DBAMolecular Sequence DataPhylogenySequence AlignmentImmunoglobulin Variable Region

Identifiers

PMID12733005

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.